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Esther Dos Santos, Ph. D. , Valérie Serazin, Ph. D

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1 Adiponectin and leptin systems in human endometrium during window of implantation 
Esther Dos Santos, Ph.D., Valérie Serazin, Ph.D., Corinne Morvan, Antoine Torre, Ph.D., Robert Wainer, Ph.D., Philippe de Mazancourt, Ph.D., Marie-Noëlle Dieudonné, Ph.D.  Fertility and Sterility  Volume 97, Issue 3, Pages e1 (March 2012) DOI: /j.fertnstert Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

2 Figure 1 Leptin and Ob-R expression in the human endometrium. (A) Leptin and Ob-R mRNA expressions. Total RNA was extracted from endometria biopsies of fertile and infertile women and then analyzed by quantitative RT-PCR. The values are the mean ± SEM and are expressed as percentages of the control value (fertile subjects). ∗P<.05; ns = nonsignificant, unpaired t test. (B) Leptin protein expression. Endometrial explants were fixed, paraffin-embedded, and stained for anti-leptin antibody as described in Materials and Methods. This figure shows one representative experiment among five separate experiments. (C) Ob-R protein expression. Cell lysates (50 μg) were extracted from endometria biopsies of fertile (CONT) and infertile (IF) women and then subjected to Western blot analysis using anti–Ob-R or anti–β-actin antibody, as described in Materials and Methods. This figure shows one representative experiment among 8–10 separate experiments. The values are the mean ± SEM and are expressed as percentages of the control value (fertile subjects). ∗P<.05; ns = nonsignificant, unpaired t test. Fertility and Sterility  , e1DOI: ( /j.fertnstert ) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

3 Figure 2 Adiponectin and AdipoR expression in the human endometrium. (A) Adiponectin and AdipoR mRNA expressions. Total RNA was extracted from endometria biopsies of fertile and infertile women and then analyzed by quantitative RT-PCR. The values are the mean ± SEM and are expressed as percentages of the control value (fertile subjects). ∗P<.05; ns = nonsignificant, unpaired t test. (B) AdipoR protein expression. Cell lysates (50 μg) were extracted from endometria biopsies of fertile (CONT) and infertile (IF) women and then subjected to Western blot analysis using anti–Ob-R or anti–β-actin antibody, as described in Materials and Methods. This figure shows one representative experiment among 8–10 separate experiments. The values are the mean ± SEM and are expressed as percentages of the control value (fertile subjects). ∗P<.05; ns = nonsignificant, unpaired t test. Fertility and Sterility  , e1DOI: ( /j.fertnstert ) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions


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