1. Image resolution in cleared tissue.
Image resolution in cleared tissue. A, The USAF test target pattern imaged with a 10× air objective on a Nikon light microscope. The lines in element 6-6 and 7-6 are ∼4.4 and 2.2 µm thick, respectively. B, The same test pattern is shown with a cleared brain (∼6 mm thick) over the pattern. The 2.2-µm-thick lines can still be resolved through the tissue. C, Analysis of dendritic spines is possible in cleared tissue using a confocal microscope with 25× oil objective. D, Axonal filopodia in the mossy fiber pathway can be observed in thick tissue sections.
Jonathan R. Epp et al. eneuro 2015;2:ENEURO
©2015 by Society for Neuroscience