1. Feedback signalling through TP53‐CDKN1A‐GADD45A‐MAPK14‐GRB2‐TGFBRII‐TGFβ induces ROS production and maintains DDR. (A–C) MRC5 cells were transfected with wtTP53 (pC‐p53), empty vector (pCDNA), siRNA as indicated, inhibitor treated or untreated (N).
Feedback signalling through TP53‐CDKN1A‐GADD45A‐MAPK14‐GRB2‐TGFBRII‐TGFβ induces ROS production and maintains DDR. (A–C) MRC5 cells were transfected with wtTP53 (pC‐p53), empty vector (pCDNA), siRNA as indicated, inhibitor treated or untreated (N). DHR fluorescence intensity (black) and γH2AX foci frequency (red) were measured in unirradiated cells and at 48 h after 20 Gy IR (M±s.e.m., n=3). *P<0.04, **P<0.01, ***P<0.001 (ANOVA/Tukey), NS, not significant. (D) Pathways connecting CDKN1A to TGFβ through MAPK14. Edge thickness: LLS for any individual interaction; edge colour: pathway LLS; node colour: log fold mRNA change in senescent versus young MRC5; arrows: genes blocked in (B) and (C); grey: most probable pathway.
João F Passos et al. Mol Syst Biol 2010;6:347
© as stated in the article, figure or figure legend