1. Volume 58, Issue 1, Pages 199-200 (January 2013)
Erratum to “Human pluripotent stem cell-derived hepatocytes support complete replication of hepatitis C virus” [J Hepatol 2012;57:246–251] 
Philip Roelandt, Susan Obeid, Jan Paeshuyse, Jolien Vanhove, Alfons Van Lommel, Yaakov Nahmias, Frederik Nevens, Johan Neyts, Catherine M. Verfaillie 
Journal of Hepatology 
Volume 58, Issue 1, Pages (January 2013)
DOI: /j.jhep
Copyright © 2012 European Association for the Study of the Liver Terms and Conditions

2. Fig. 1
Hepatocyte differentiation from human embryonic stem cells with adapted protocol. (A) Growth factor combinations used [Roelandt P, Pauwelyn KA, Sancho-Bru P, Subramanian K, Bose B, Ordovas L, et al. Human embryonic and rat adult stem cells with primitive endoderm-like phenotype can be fated to definitive endoderm, and finally functional hepatocyte-like cells. PLoS ONE 2010;5:e12101] compared with adapted protocol used in the current study. Final concentrations were Activin-A 100ng/ml, Wnt3a 50ng/ml, BMP4 50ng/ml, FGF1 50ng/ml and HGF 20ng/ml. (B) Differentiated progeny of the hESC cell line were harvested on day 28 of differentiation using the published protocol or the new protocol. Expression of hepatoblast/hepatocyte marker genes was evaluated by RT-qPCR. Data are provided as fold change compared with levels on day 0. Data are shown as mean±SD (n>3). (C) After 28days, cells that had been differentiated using the new protocol were fixed and immunostaining was performed for ALB/AFP and ALB/CYP3A4/5. (Representative example of n=3.) (D–F) After 28days of differentiation, the secretion of albumin (D), the production of urea after addition of ammonia (E), and the CYP3A4 activity, un-induced and after induction with phenobarbital (F) were compared using cultures obtained using the published protocol and the new protocol (n=3).
Journal of Hepatology  , DOI: ( /j.jhep )
Copyright © 2012 European Association for the Study of the Liver Terms and Conditions

3. Fig. 2
HCV receptors. (A) RT-qPCR analysis of gene expression in day 20 differentiated hESC progeny used for inoculation with HCV, primary hepatocytes and HuH7.5.1 cells, compared to the housekeeping gene GAPDH. Data are shown as mean±SD (n=3). (B) Immunostaining showing co-localization of SR-B1 with HNF4α, and of CD81 with ALB in hESC progeny on day 20. Size bars 20μm. Representative example of n=3.
Journal of Hepatology  , DOI: ( /j.jhep )
Copyright © 2012 European Association for the Study of the Liver Terms and Conditions

4. Fig. 3
HCV infection. (A) Immunostaining of NS5A in ALB+ and CYP3A4/5+ cells. (Representative example of n=3.) (B) Quantification of HCV positive strand RNA (by RT-qPCR) in (i) intracellular extracts of hESC progeny, (ii) supernatants of hESC progeny and (iii) supernatants of HuH7.5.1 cells, 10days after HCV inoculation (n=5). Levels following mock infection were 10−5fg. (C) Inhibition of RNA replication in hESC progeny (i) untreated, (ii) treated with 10μM HCV-796 (n=4), (iii) treated with 5μM Debio 025 (n=2) and (iv) treated with 1μM VX-950 (n=4) in hESC progeny and HuH7.5.1 cells.
Journal of Hepatology  , DOI: ( /j.jhep )
Copyright © 2012 European Association for the Study of the Liver Terms and Conditions

5. Fig. 4
HCV egress. (A) Levels of albumin, ApoB100 and HCV core protein in supernatants of non-infected hESC progeny and hESC progeny at different time points after inoculation (n=3). (B) Infection of HuH7.5.1 with mock (a), HCV (b), supernatants collected 4days post-infection of hESC-progeny with HCV (c), 8days post-infection (d) and 11days post-infection (e). Representative for n=2.
Journal of Hepatology  , DOI: ( /j.jhep )
Copyright © 2012 European Association for the Study of the Liver Terms and Conditions