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Locally elevated leukemia inhibitory factor in the inflamed fallopian tube resembles that found in tubal pregnancy  Yin Fen Ji, M.D., Li You Chen, M.M.,

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Presentation on theme: "Locally elevated leukemia inhibitory factor in the inflamed fallopian tube resembles that found in tubal pregnancy  Yin Fen Ji, M.D., Li You Chen, M.M.,"— Presentation transcript:

1 Locally elevated leukemia inhibitory factor in the inflamed fallopian tube resembles that found in tubal pregnancy  Yin Fen Ji, M.D., Li You Chen, M.M., Kai Hong Xu, M.M., Ji Fen Yao, M.M., Yi Fu Shi, M.D.  Fertility and Sterility  Volume 91, Issue 6, Pages (June 2009) DOI: /j.fertnstert Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

2 Figure 1 Expression of LIF protein in epithelial cell of human fallopian tube determined by immunohistochemistry. The protein was mainly localized in epithelial cell and faint in stromal cell. Immunostaining of LIF in implantation site (A, original magnification ×400) was more intense than in corresponding nonimplantation site (B, original magnification ×400) and also stronger in the group with chronic inflammation (C, original magnification ×400) than in the normal group (D, original magnification ×400). The right corner of E (original magnification ×200) is proximate to the implantation site. To clearly show the different expression due to different distance from implantation site, we cannot include the villus and the different expression epithelium in the same illustration. The nearer to the anchoring villus, the stronger staining of LIF in epithelium was observed (E). Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

3 Figure 2 Box and whisker plot of LIF protein in the four groups. (A) H-score values of LIF expression in epithelium of the fallopian tubes determined by immunohistochemistry. (B) LIF/actin ratio determined by Western blot analysis. Values are median (horizontal bar), 25th/75th interquartile ranges (box), and maximum/minimum values (whiskers). Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

4 Figure 3 Expression of LIF protein assessed by Western blotting in human fallopian tubes. β-Actin was used as a control to assess the amount of protein in each sample. The anti-LIF antibody detected a predominant band at 45 kDa. Chronic salpingitis tissues (1, 4), normal fallopian tubes (2, 3), fallopian tubal tissues in implantation site (5, 7), fallopian tubal tissues in nonimplantation site (6, 8). Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions


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