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Published byMeredith Sutton Modified over 5 years ago
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N H N H Figure S1 parp-1 +/+ parp-1 -/- HIF-2 DAPI
Fig S1. Nuclear HIF-2 accumulation by hypoxia is reduced in parp-1 -/- immortalized MEFs. Immunofluorescence staining of parp-1 +/+ and parp-1 -/- immortalized MEFs incubated in normoxia (N) or hypoxia (H, 16 hours at 1 % O2). Representative images from several independent experiments are shown. 1
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Figure S2 H H Control PARP-1 siRNA: Control siRNA: HIF-2α siRNA: Control HIF-1α PARP-1 HIF-2α HIF-1α β-Actin β-Actin β-Actin Figure S2. Validation of silencing efficacy: siRNA of PARP-1, HIF-2 or HIF-1. (Cells were transfected with the correspondig siRNAs and 48h later, the expression levels of the targeted protein was evaluated by WB. HIF expression was analyzed after 16 hours of exposure to hypoxia (1% O2). 2
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Figure S3 15 75 parp-1+/+ 15 Liver Kidney Brain parp-1-/- 10 50 10 Epo mRNA expression (fold induction) * 5 25 5 ** N H N H N H Figure S3. EPO mRNA levels were determined by Q-PCR from liver, kidney and brain . The level of mRNA in each sample was expressed as fold of the value in control wild-type mice. Numeric value represents mean of hypoxic induction in each genotype at 8 hours, respectively. (*) and (**) indicate p< 0.05 and p<0.001, with respect to wild-type mice. 3
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