1. EphB4 stimulation does not cause vessel regression
EphB4 stimulation does not cause vessel regression Mouse limb muscles were implanted with the V‐high myoblast clone, while treating animals systemically with ephrinB2‐Fc or control Fc protein. As a positive control for vessel regression, mice implanted with the V‐high clone were treated with the potent VEGF blocker aflibercept (V‐high + aflibercept). Immunofluorescence staining for endothelium (CD31, green), basal lamina (laminin; lam, red), and nuclei (DAPI, blue) showed that ephrinB2‐Fc treatment did not cause any vessel regression, as all basal laminas were associated with endothelial tubes. By contrast, after aflibercept treatment widespread vessel regression was evident through the detection of empty sleeves of basal lamina (white arrows) devoid of endothelium, remnants of disappeared vessels. Scale bar: 25 μm.
Elena Groppa et al. EMBO Rep. 2018;embr
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