1. Protein delivery: DNA nanostructures and cell-surface targeting
Harvard iGEM
August 27, 2006

2. The Machine
Goal: Future modularized drug delivery
target cell
protein

3. DNA Nanostructures Overview
Can design DNA double helices to stick together and form interesting structures.
Dr. Ned Seeman, NYU
Dr. William Shih, Harvard
Paul Rothemund, Caltech
A 1.7-kilobase single-stranded DNA that folds into a nanoscale octahedron
WILLIAM M. SHIH, JOEL D. QUISPE & GERALD F. JOYCE
Nature 427, 618ﾐ621 (2004); doi: /nature02307

4. Motivation: Why DNA? Fascinating area of research The power of DNA
Watson-Crick base pairing is enormously strong
Self-assembly
Highly programmable, designable
Specificity - targeting to cells

5. Design Details

6. Design Details: Scaffolded Oragami

7. Design Details: Scaffolded Oragami

8. Design Details: Scaffolded Oragami

9. Design Details: Positional Control

10. Design Details: Positional Control

11. Design Details: Positional Control

12. Design Details: Positional Control

13. Design Details: Positional Control

14. Design Details: Positional Control

15. Progress Built a number of barrel designs Exciting EM Images
Purifying Nanostructures (nearly there after 1 month of trials)

16. Exciting EM Images

17. Exciting EM Images

18. EM Images (snakes on a grid)
c5.0 barrel (10 nM), 0.7% uranyl formate
Appear to be lining up end to end, probably because of the stain
Images courtesy Shawn Douglas

19. To be continued
protease
Can a protein be protected from protease if attached inside the box?
Lid attachment
Lid removal
protein
protease
protein

20. Acknowledgements
Harvard TFs - Shawn Douglas, Nick Stroustrup, Chris Doucette
Harvard advisers - Dr. William Shih, Dr. George Church, Dr. Pamela Silver, Dr. Alain Viel, Dr. Jagesh Shah, Dr. Radhika Nagpal
iGEM ambassadors
iGEM directors