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Stacie M. Jones, MD, Chiara F. Magnolfi, MD, Sara K. Cooke, SB, Hugh A

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Presentation on theme: "Stacie M. Jones, MD, Chiara F. Magnolfi, MD, Sara K. Cooke, SB, Hugh A"— Presentation transcript:

1 Immunologic cross-reactivity among cereal grains and grasses in children with food hypersensitivity 
Stacie M. Jones, MD, Chiara F. Magnolfi, MD, Sara K. Cooke, SB, Hugh A. Sampson, MD  Journal of Allergy and Clinical Immunology  Volume 96, Issue 3, Pages (September 1995) DOI: /S (95) Copyright © 1995 Mosby, Inc. Terms and Conditions

2 FIG. 1 Taxonomic relationship between cereal grains and grasses of family Poaceae. Journal of Allergy and Clinical Immunology  , DOI: ( /S (95) ) Copyright © 1995 Mosby, Inc. Terms and Conditions

3 FIG. 2 Amido black staining of the SDS-PAGE sequence for cereal grains. mwm, Molecular weight markers; w, wheat; r, rye; br, barley; o, oat; g, gluten; ri, rice; c, corn. Journal of Allergy and Clinical Immunology  , DOI: ( /S (95) ) Copyright © 1995 Mosby, Inc. Terms and Conditions

4 FIG. 3 Amido black staining of the SDS-PAGE sequence for cereal grains and grasses. mwm, Molecular weight markers; w, wheat; r, rye; o, oat; t, timothy; m, meadow fescue; b, Bermuda; s, sweet vernal. Journal of Allergy and Clinical Immunology  , DOI: ( /S (95) ) Copyright © 1995 Mosby, Inc. Terms and Conditions

5 FIG. 4 Immunoblot panel probed with IgE myeloma protein.
Journal of Allergy and Clinical Immunology  , DOI: ( /S (95) ) Copyright © 1995 Mosby, Inc. Terms and Conditions

6 FIG. 5 Immunoblot probed with patient serum from group 1. Numerous cross-reactive bands were identified among grains, despite the fact that this patient was allergic to only two of the grains (wheat and barley). Journal of Allergy and Clinical Immunology  , DOI: ( /S (95) ) Copyright © 1995 Mosby, Inc. Terms and Conditions

7 FIG. 6 Immunoblot of grains and grasses probed with serum from the patient in group 1 who was allergic to wheat, barley, and grasses. Journal of Allergy and Clinical Immunology  , DOI: ( /S (95) ) Copyright © 1995 Mosby, Inc. Terms and Conditions

8 FIG. 7 Representative immunoblot from a patient only allergic to wheat in group 2 who had negative SPT responses to grasses. Journal of Allergy and Clinical Immunology  , DOI: ( /S (95) ) Copyright © 1995 Mosby, Inc. Terms and Conditions

9 FIG. 8 Representative immunoblot from a patient in group 3 who was allergic to grasses but no cereal grains. Journal of Allergy and Clinical Immunology  , DOI: ( /S (95) ) Copyright © 1995 Mosby, Inc. Terms and Conditions

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