1. Volume 95, Issue 11, Pages 5476-5486 (December 2008)
Characterization of Protein Dynamics in Asymmetric Cell Division by Scanning Fluorescence Correlation Spectroscopy 
Zdeněk Petrášek, Carsten Hoege, Alireza Mashaghi, Thomas Ohrt, Anthony A. Hyman, Petra Schwille 
Biophysical Journal 
Volume 95, Issue 11, Pages (December 2008)
DOI: /biophysj
Copyright © 2008 The Biophysical Society Terms and Conditions

2. Figure 1
Diffusion coefficients of GFP:PAR-2 (A and C) and NMY-2:GFP (B and D) in the cytosol. The measurement volume was positioned into different parts of the cytosol, while focused into the midplane of the embryo (A and B), and the measured autocorrelation curves were fitted to Eq. 2. The distributions of the obtained diffusion coefficients D are shown in panels C (GFP:PAR-2) and D (NMY-2:GFP). (Scale bar, 10μm. a, anterior; p, posterior.)
Biophysical Journal  , DOI: ( /biophysj )
Copyright © 2008 The Biophysical Society Terms and Conditions

3. Figure 2
Nonuniform fluorescence pattern of GFP:PAR-2 (A and C) and NMY-2:GFP (B and D) on the cortex. The objective was focused near the coverslip onto the flattened part of the embryo. The circles in panels A and B indicate the scan path for sFCS measurements. (C and D) The fluctuating fluorescence intensity recorded in the sFCS measurement is displayed in a two-dimensional plot, where the horizontal axis corresponds to one revolution (scan period T), and the vertical axis to subsequent revolutions during the course of measurement (from top to bottom). The columns in the plot then show the fluorescence fluctuations at individual positions along the scanned circle. (C) GFP:PAR-2, D: NMY-2:GFP. (Scale bar, 10μm. a, anterior; p, posterior.)
Biophysical Journal  , DOI: ( /biophysj )
Copyright © 2008 The Biophysical Society Terms and Conditions

4. Figure 3
The experimental fluorescence autocorrelations from sFCS measurements (shaded). The amplitudes of the peaks obtained from fits to Eq. 7 are indicated by solid dots. (A) GFP:PAR-2, (B) NMY-2:GFP.
Biophysical Journal  , DOI: ( /biophysj )
Copyright © 2008 The Biophysical Society Terms and Conditions

5. Figure 4
Comparison of sFCS autocorrelations of GFP:PAR-2 (black) and NMY-2:GFP (thick gray) on the cortex and FCS autocorrelations of GFP:PH (thin gray) on the membrane from measurements in several embryos. The sFCS autocorrelations are formed by the fitted amplitudes of the peaks as shown in Fig. 3.
Biophysical Journal  , DOI: ( /biophysj )
Copyright © 2008 The Biophysical Society Terms and Conditions

6. Figure 5
Comparison of a typical sFCS autocorrelation (blue) of GFP:PAR-2 (A) and NMY-2:GFP (B) with several simple models of transport: uniform flow (green), binding/dissociation (magenta), normal diffusion (black), anomalous diffusion (gray), normal two-component diffusion (red), and flow with a Gaussian distribution of speeds, centered at v=0:p(v)∼exp(−v2/(2σv2)) (cyan). The parameters τi are the characteristic time constants of the relevant process.
Biophysical Journal  , DOI: ( /biophysj )
Copyright © 2008 The Biophysical Society Terms and Conditions

7. Figure 6
sFCS autocorrelation of GFP:PAR-2 displayed in spatiotemporal representation, and fits to three different models. The value x is the spatial and τ the temporal correlation coordinate. (A) The spatiotemporal autocorrelation. (B) The spatiotemporal autocorrelation normalized to the maximum at each τ value to emphasize the spatial broadening. (C and F) Fit to a one-component diffusion model and the residuals of the fit (χ2=4.61×10−6). The white rectangle denotes the fitting range. (D and G) Fit to a two-component diffusion model and the fit residuals (χ2=3.99×10−6). (E and H) Fit to a model with one diffusion and one binding/dissociation components, and the fit residuals (χ2=4.37×10−6). The correlation coordinate x (corresponding to the distance along the scanned circle) can be equivalently expressed by the scan phase φ, or by the time from the peak maximum τ. These three coordinates are related to each other in the following way: x=2R sin(ωτ/2), φ=ωτ.
Biophysical Journal  , DOI: ( /biophysj )
Copyright © 2008 The Biophysical Society Terms and Conditions

8. Figure 7
sFCS autocorrelation of NMY-2:GFP displayed in spatiotemporal representation. (A) The spatiotemporal autocorrelation. (B) The spatiotemporal autocorrelation normalized to the maximum at each τ.
Biophysical Journal  , DOI: ( /biophysj )
Copyright © 2008 The Biophysical Society Terms and Conditions