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Cell Biology Overview II. Membranes – How Things Get in and Out of Cells A. Membrane Structure 1. phospholipids POLAR END (react with water) NON-POLAR.

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Presentation on theme: "Cell Biology Overview II. Membranes – How Things Get in and Out of Cells A. Membrane Structure 1. phospholipids POLAR END (react with water) NON-POLAR."— Presentation transcript:

1 Cell Biology Overview II. Membranes – How Things Get in and Out of Cells A. Membrane Structure 1. phospholipids POLAR END (react with water) NON-POLAR END (hyrophobic)

2 Cell Biology Overview II. Membranes – How Things Get in and Out of Cells A. Membrane Structure 2. proteins and carbohydrates

3 Cell Biology Overview II. Membranes – How Things Get in and Out of Cells A. Membrane Structure B. Membrane Function 1. semi-permeable barrier Aqueous Solution (outside cell) dissolved ions dissolved polar molecules suspended non-polar (lipid soluble) Aqueous Solution (inside cell) dissolved ions dissolved polar molecules suspended non-polar (lipid soluble)

4 Cell Biology Overview II. Membranes – How Things Get in and Out of Cells A. Membrane Structure B. Membrane Function 1. semi-permeable barrier 2. transport Net diffusion Net diffusion equilibrium

5 Cell Biology Overview II. Membranes – How Things Get in and Out of Cells A. Membrane Structure B. Membrane Function 1. semi-permeable barrier 2. transport – facilitated diffusion

6 Cell Biology Overview II. Membranes – How Things Get in and Out of Cells A. Membrane Structure B. Membrane Function 1. semi-permeable barrier 2. transport - osmosis

7 Cell Biology Overview II. Membranes – How Things Get in and Out of Cells A. Membrane Structure B. Membrane Function 1. semi-permeable barrier 2. transport – active transport

8 Cell Biology Overview II. Membranes – How Things Get in and Out of Cells A. Membrane Structure B. Membrane Function 1. semi-permeable barrier 2. transport 3. metabolism (enzymes nested in membrane) 4. signal transduction

9 Cell Biology Overview II. Membranes – How Things Get in and Out of Cells A. Membrane Structure B. Membrane Function 1. semi-permeable barrier 2. transport 3. metabolism (enzymes nested in membrane) 4. signal transduction 5. cell-cell binding 6. cell recognition 7. cytoskeleton attachment

10 Cellular Respiration

11 III. Cellular Respiration Overview:
Some trapped in bonds between ADP + P ATP NAD + H+ NADH Some E lost (heat) Organic Molecules: C—C—C—C Break C—C bonds release E Proteins, Carbohydrates, Fats, Nucleic Acids C C C C (These are CO2 molecules)

12 III. Cellular Respiration Overview:
Some trapped in bonds between ADP + P ATP NAD + H+ NADH Some E lost (heat) Organic Molecules: C—C—C—C Break C—C bonds release E Proteins, Carbohydrates, Fats, Nucleic Acids C C C C (These are CO2 molecules) Break NADH ADP + P Make ATP NAD, H+

13 III. Cellular Respiration
Overview: Focus on core process… Glucose metabolism GLYCOLYSIS

14 Glucose 2 pyruvate C6H12O6 2 C3 III. Cellular Respiration Overview:
1. Glycolysis: - All cells do this! (very primitive pathway) - Occurs in the cytoplasm of all cells - Occurs in presence OR absence of oxygen gas. Glucose C6H12O6 2 pyruvate 2 C3

15 Glucose 2 pyruvate C6H12O6 2 C3 III. Cellular Respiration Overview:
1. Glycolysis: - Energy in 2 ATP is used to ‘activate’/start reaction 2 ATP 2 ADP + P Glucose C6H12O6 2 pyruvate 2 C3

16 Glucose 2 pyruvate C6H12O6 2 C3 III. Cellular Respiration Overview:
1. Glycolysis: - Energy in 2 ATP is used to ‘activate’/start reaction - breaking the C-C bond in glucose releases e- /Energy - electrons accepted by NAD NAD- +H NADH NAD NADH 2 ATP 2 ADP + P Glucose C6H12O6 2 pyruvate 2 C3 4 ADP + P ATP

17 Anaerobic Respiration
III. Cellular Respiration Overview: GLYCOLYSIS PYRUVATE METABOLISM Oxygen Absent? Oxygen Present? Anaerobic Respiration Or Fermentation Aerobic Respiration SOME ATP MORE ATP

18 C3 C2-CoA + CO2 III. Cellular Respiration Overview: Glycolysis
Anaerobic Respiration C C2-CoA + CO2 Pyruvate Acetyl-CoA NAD NADH

19 Incomplete Citric Acid Cycle C5 + CO2
III. Cellular Respiration Overview: Glycolysis Anaerobic Respiration C C2-CoA + CO2 CoA NAD NADH NAD NADH C6 C4 Incomplete Citric Acid Cycle C5 + CO2

20 2. Anaerobic Respiration
Electron Transport Chain across a membrane to convert energy harvested in NADH into bonds in ATP. OUTER Intermembrane (periplasmic) space INNER e- NADH NAD + H+ STEP 1: NADH gives up electron to an electron acceptor protein in the membrane, splitting into NAD and H+ ions. NAD is RECYCLED at this step, so GLYCOLYSIS can continue!

21 2. Anaerobic Respiration
Electron Transport Chain across a membrane to convert energy harvested in NADH into bonds in ATP. OUTER Intermembrane (periplasmic) space H+ H+ e- INNER e- NADH NAD + H+ H+ Step 2: The electron is passed from protein to protein, across the inner membrane. This movement of negative charge draws H+ ions across through protein channels… H+ build up in the intermembrane space, creating a CHARGE DIFFERENTIAL.

22 2. Anaerobic Respiration
Electron Transport Chain across a membrane to convert energy harvested in NADH into bonds in ATP. OUTER Intermembrane (periplasmic) space H+ H+ H+ e- INNER e- ATP synthase NADH NAD ADP + P ATP Step 3: The charge differential is electric potential energy. Eventually, the differential is so great that H+ ions flow back through the membrane. The energy in this ‘current’ of charged particles is used to add P + ADP  ATP.

23 2. Anaerobic Respiration
Electron Transport Chain across a membrane to convert energy harvested in NADH into bonds in ATP. OUTER Intermembrane (periplasmic) space e- INNER ADP + P ATP e- NADH NAD S- H2S S H+ H+ Step 4: The electron(s) are accepted by a ‘final electron acceptor’. In anaerobic respiration, this is CO2, NO3, Fe, or S. And the anion formed reacts with H+ ions. THUS, ENERGY IN NADH IS USED TO MAKE BONDS IN ATP.

24 III. Cellular Respiration Overview: Glycolysis Anaerobic Respiration
Ethanol Fermentation: Some bacteria, plants, yeasts Lactate Fermentation: Some bacteria, animals

25 Anaerobic Respiration
III. Cellular Respiration Overview: GLYCOLYSIS PYRUVATE METABOLISM Oxygen Absent? Oxygen Present? Anaerobic Respiration Or Fermentation Aerobic Respiration SOME ATP MORE ATP

26 III. Cellular Respiration Overview: Glycolysis Anaerobic Respiration
Occurs in: Aerobic bacteria Aerobic Archaea All Eukaryotes (in the mitochondria descended from aerobic bacteria!) GLYCOLYSIS OCCURS IN THE CYTOPLASM. THAT’S WHERE THE PYRUVATES ARE PRODUCED.

27 C3 C2-CoA + CO2 III. Cellular Respiration Overview: Glycolysis
Anaerobic Respiration C C2-CoA + CO2 Pyruvate Acetyl-CoA NAD NADH “Gateway Step” In Eukaryotes

28 CoA – C2 C6 C4 C5 + CO2 + CO2 CoA REVIEW – ANAEROBIC RESPIRATION
Incomplete Citric Acid Cycle CoA – C2 C4 C6 C5 + CO2 NAD NADH + CO2 FAD FADH2 ADP + P ATP CoA (oxaloacetate) NAD NADH

29 CoA – C2 C6 C4 C5 + CO2 + CO2 CoA Aerobic Respiration
Citric Acid (“Krebs”) Cycle CoA – C2 C4 C6 C5 + CO2 NAD NADH + CO2 FAD FADH2 ADP + P ATP CoA (oxaloacetate) NAD NADH The C5 molecule is recycled into oxaloacetate (C4), with the release of even MORE ENERGY trapped in ATP, NADH, and FADH

30 2. Aerobic Respiration Electron Transport Chain across a membrane to convert energy harvested in NADH into bonds in ATP. OUTER Intermembrane (periplasmic) space H+ H+ H+ H+ H+ H+ e- INNER e- e- ADP + P ATP NADH NAD + H+ O2 2 O-- 2 H2O FADH FAD + H+ 2H+ 2H+ SAME THING: Electrons transferred, H+ ions pulled across membrane, and flood back across creating ATP. But O2 is the final electron acceptor, making WATER!! MORE electrons transferred, stronger ‘pull’ exerted by oxygen, more ATP made.

31 Alcohol fermentation CO2 2 NADH 2 NAD+ 2 Acetaldehyde 2 ATP 2 ADP + 2
LE 9-17a CO2 2 NADH 2 NAD+ 2 Acetaldehyde 2 ATP 2 ADP + 2 i 2 Pyruvate 2 Ethanol Glucose Glycolysis P 2 + 2 H+ Alcohol fermentation

32 Lactic acid fermentation
LE 9-17b + 2 H+ 2 NADH 2 NAD+ 2 ATP 2 ADP + 2 P 2 Pyruvate Glucose Glycolysis i Lactate 2 Lactate Lactic acid fermentation

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