Presentation is loading. Please wait.

Presentation is loading. Please wait.

Development of a 3D tissue model and in vivo live imaging during dermal maturation Development of a 3D tissue model and in vivo live imaging during dermal.

Published byθάλασσα Μέλιοι Modified over 5 years ago

Similar presentations

Presentation on theme: "Development of a 3D tissue model and in vivo live imaging during dermal maturation Development of a 3D tissue model and in vivo live imaging during dermal."— Presentation transcript:

1 Development of a 3D tissue model and in vivo live imaging during dermal maturation
Development of a 3D tissue model and in vivo live imaging during dermal maturation A(x,y) Cross‐section of the computational model simulation of the dermal maturation process at the indicated Monte Carlo step (MCS). Colour code indicates epidermis (green), proliferating fibroblasts (blue), quiescent fibroblasts (yellow), ECM (grey) and adipocytes (brown) (see accompanying Movie EV1).BAbundance of the cell populations during the course of the simulation.CQuantification of fibroblast proliferation and ECM density in the simulation.DLive imaging of adult PDGFRαH2BEGFP mouse back skin. Representative time‐lapse images of adult PDGFRαH2BEGFP (green) dermis with collagen shown as second harmonic generation (SHG) in purple at indicated imaging time points. Scale bar, 100 μm (see accompanying Movie EV2).E–GIn silico lineage tracing of fibroblasts. (E) Labelling of fibroblasts close to the epidermis at indicated MCS. (F) Labelling of fibroblasts in the middle of the dermis at indicated MCS. (G) Labelling of fibroblasts close to the inside of the body at indicated MCS. Emanuel Rognoni et al. Mol Syst Biol 2018;14:e8174 © as stated in the article, figure or figure legend

Download ppt "Development of a 3D tissue model and in vivo live imaging during dermal maturation Development of a 3D tissue model and in vivo live imaging during dermal."

Similar presentations

Hydration profiles of GLIC′s pore in WT and Ser6′ mutant simulations.

Hydration profiles of GLIC′s pore in WT and Ser6′ mutant simulations.
Tuning the response of the DPI

Tuning the response of the DPI
A stochastic feedback loop model predicts the kinetics of DDR and growth arrest at the single cell level. A stochastic feedback loop model predicts the.

A stochastic feedback loop model predicts the kinetics of DDR and growth arrest at the single cell level. A stochastic feedback loop model predicts the.
Bifurcation diagrams showing quasi‐steady‐state solutions and simulation of models. Bifurcation diagrams showing quasi‐steady‐state solutions and simulation.

Bifurcation diagrams showing quasi‐steady‐state solutions and simulation of models. Bifurcation diagrams showing quasi‐steady‐state solutions and simulation.
Analysis of in silico flux changes along the exponential growth phase: (A) In silico flux changes from 24 to 36 h, from 36 to 48 h, and from 48 to 60 h.

Analysis of in silico flux changes along the exponential growth phase: (A) In silico flux changes from 24 to 36 h, from 36 to 48 h, and from 48 to 60 h.
Dual allele labeling reveals a trans‐acting source for extrinsic noise

Dual allele labeling reveals a trans‐acting source for extrinsic noise
Notch levels in niche cells

Notch levels in niche cells
The TBON model. The TBON model. (A) Representative confocal images of E14Tg2A cells stained for Tcf3 (green), Nanog (red), Oct4 (magenta), and total β‐catenin.

The TBON model. The TBON model. (A) Representative confocal images of E14Tg2A cells stained for Tcf3 (green), Nanog (red), Oct4 (magenta), and total β‐catenin.
Skin proliferation kinetics and in vivo fibroblast lineage tracing during dermal maturation (related to Fig 4)‏ Skin proliferation kinetics and in vivo.

Skin proliferation kinetics and in vivo fibroblast lineage tracing during dermal maturation (related to Fig 4)‏ Skin proliferation kinetics and in vivo.
Dose response of pAGA1 and pFIG1 induction

Dose response of pAGA1 and pFIG1 induction
Skin Cell Heterogeneity in Development, Wound Healing, and Cancer

Skin Cell Heterogeneity in Development, Wound Healing, and Cancer
Dual LGR5 and KI67 knock‐in PDOs enable separation of quiescent and cycling LGR5+ CRC cells Dual LGR5 and KI67 knock‐in PDOs enable separation of quiescent.

Dual LGR5 and KI67 knock‐in PDOs enable separation of quiescent and cycling LGR5+ CRC cells Dual LGR5 and KI67 knock‐in PDOs enable separation of quiescent.
Luminex phosphorylation measurements are reproducible and have broad dynamic range Individual difference in insulin‐induced phosphorylation of ERK measured.

Luminex phosphorylation measurements are reproducible and have broad dynamic range Individual difference in insulin‐induced phosphorylation of ERK measured.
cuf2Δ and fzr1Δ cells show a defect in meiotic termination.

cuf2Δ and fzr1Δ cells show a defect in meiotic termination.
Can I color yellow?. Can I color yellow?

Can I color yellow?. Can I color yellow?
TFEB stimulates lysosomal–autophagosomal fusion and clearance

TFEB stimulates lysosomal–autophagosomal fusion and clearance
Genomic profiling of fitness in periodic salt stress

Genomic profiling of fitness in periodic salt stress
Thomas Yang Sun, Ann M. Haberman, Valentina Greco 

Thomas Yang Sun, Ann M. Haberman, Valentina Greco 
Stromal fibromuscular AR regulates epithelium proliferation, ECM remodelling, neovasculature formation and immune cell infiltration in Pten deficient mice.

Stromal fibromuscular AR regulates epithelium proliferation, ECM remodelling, neovasculature formation and immune cell infiltration in Pten deficient mice.
Dermal architecture is defined by an inverse correlation between fibroblast proliferation and ECM deposition Dermal architecture is defined by an inverse.

Dermal architecture is defined by an inverse correlation between fibroblast proliferation and ECM deposition Dermal architecture is defined by an inverse.

Similar presentations

Ads by Google