1. Volume 24, Issue 6, Pages 1100-1105 (June 2016)
Recombinant AAV Integration Is Not Associated With Hepatic Genotoxicity in Nonhuman Primates and Patients 
Irene Gil-Farina, Raffaele Fronza, Christine Kaeppel, Esperanza Lopez-Franco, Valerie Ferreira, Delia D'Avola, Alberto Benito, Jesus Prieto, Harald Petry, Gloria Gonzalez-Aseguinolaza, Manfred Schmidt 
Molecular Therapy 
Volume 24, Issue 6, Pages (June 2016)
DOI: /mt
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2. Figure 1
Standard and multiplex (M)-LAM-PCR comparison analysis and retrieved vector integration sites (IS). (a) DNA was isolated from tissue samples and analyzed by standard LAM-PCR, that uses one primer set located within the inverted terminal repeats proximity, and M-LAM-PCR, where a total of five primer sets covering the whole vector are employed. Black arrows denote location of the individual LAM-PCR primer sets on the vector genome. (b) Vector IS identified in nonhuman primates tissues (▪ liver, ▪ adrenal gland, ▪ spleen) and human liver (▪) were mapped to their respective genome. Integration clusters found (o) and the hepatocellular carcinoma-related genes recently described (▪) are shown. LAM, linear amplification-mediated; PCR, polymerase chain reaction.
Molecular Therapy  , DOI: ( /mt )
Copyright © 2016 American Society of Gene & Cell Therapy Terms and Conditions

3. Figure 2
Chromosomal distribution of the retrieved integration sites (IS). The percentage of IS identified on each chromosome was determined in the different nonhuman primates tissues (a) and human liver biopsies (b). Chromosomal distributions from synthetic data sets of random IS are shown for comparison.
Molecular Therapy  , DOI: ( /mt )
Copyright © 2016 American Society of Gene & Cell Therapy Terms and Conditions

4. Figure 3
Analysis of vector rearrangements. Among reads bearing a single vector fragment (1 Fragment), we also detected concatemeric structures containing up to 8 different rearranged vector fragments (2 to 8 Fragments).
Molecular Therapy  , DOI: ( /mt )
Copyright © 2016 American Society of Gene & Cell Therapy Terms and Conditions