1. Ex. 16: Nitrate Respiration (Nitrate Reduction Test)
Objectives?

2. Vocabulary Oxidation – reduction reactions Reductase
Nitrate vs. Nitrite
Denitrification

3. Nitrate Reduction and Denitrification
nitrite reductase
nitrate reductase
Some bacteria only produce nitrate reductase, other bacteria also produce nitrite reductase

4. Day 1: Materials needed per table: Nitrate broth tubes (4)
1 sterile Q-tip
Three pure cultures needed per table:
Alcaligenes faecalis (negative)
Proteus vulgaris (positive for nitrite)
Pseudomonas aeruginosa (denitrifier)
Each student takes responsibility to inoculate one of the nitrate broth tubes