1. Co‐expression of Sorcs1 and Sorcs3 in the hypothalamus
Co‐expression of Sorcs1 and Sorcs3 in the hypothalamus A, BTranscript levels for Sorcs1 (A) and Sorcs3 (B) in the indicated mouse tissues were assessed by quantitative (q) RT–PCR. Expression levels in the hippocampus were set to 1 (n = 3 mice/group, 10 weeks of age).CComparison of Sorcs1 and Sorcs3 transcript levels using qRT–PCR in hypothalami of WT mice. Identical amplification efficiency for both gene expression assays was validated in a separate experiment (n = 5 mice/group).DTranscript levels for Sorcs1 and Sorcs3 as assessed by qRT–PCR in hypothalami from mice with single Sorcs1 (S1 KO) or Sorcs3 (S3 KO) deficiencies. S3 KO mice show a compensatory increase in Sorcs1 expression compared to WT controls. Expression in WT was set to 1 (n = 5–10 mice/group).EIn situ hybridization (ISH) for Sorcs1 and Sorcs3 on coronal brain sections indicating expression of both receptors in cerebral cortex and in various nuclei of the hypothalamus (Arc: arcuate nucleus; VMH: ventromedial nucleus; DMH: dorsomedial nucleus). ISH for Agrp and Npy on adjacent sections was used as controls for identification of the arcuate nucleus. For each gene, the lower panel represents a higher magnification of the hypothalamus area (marked in the overview micrograph of ISH for Sorcs1). Scale bar: 500 μm; n = 3 mice.Data information: Data in (A–D) are shown as mean ± SD and were analyzed using a two‐tailed unpaired t‐test. **P < 0.01, ***P < 0.001.
Aygul Subkhangulova et al. EMBO Rep. 2018;embr
© as stated in the article, figure or figure legend