1. Impact of growth phase on the Escherichia coli meltome and proteome
Impact of growth phase on the Escherichia coli meltome and proteome Melting temperatures (Tm) of proteins in exponential and transition to stationary growth phases. Proteins highlighted in orange indicate significantly different melting behavior.Protein abundance in exponential and transition to stationary growth phases, as measured by the top3 intensity corresponding to the lowest temperature (see “Materials and Methods”). Proteins highlighted in orange indicate significantly different levels. Proteins were considered not detectable (n.d.) in one condition, if absent in three replicates in that condition, but detectable by at least three unique peptides in at least two replicates in the other condition.Respiratory activity in exponential and stationary cells determined as the conversion of triphenyltetrazolium chloride to triphenylformazan during the same time and normalized by OD (˜number of cells). n = 3; error bars represent standard deviation; **P < 0.01, Student's t‐test.
André Mateus et al. Mol Syst Biol 2018;14:e8242
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