1. Mevastatin inhibits proliferation of rat ovarian theca–interstitial cells by blocking the mitogen-activated protein kinase pathway 
Jakub Kwintkiewicz, Ph.D., Nastaran Foyouzi, M.D., Piotr Piotrowski, M.D., Ph.D., Izabela Rzepczynska, M.Sc., Antoni J. Duleba, M.D. 
Fertility and Sterility 
Volume 86, Issue 4, Pages (October 2006)
DOI: /j.fertnstert
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

2. FIGURE 1
(A) Effect of mevastatin in the absence and in the presence of serum on proliferation of theca–interstitial cells assessed by incorporation of radiolabeled thymidine; the cells were incubated for 48 hours without additives (control), with mevastatin (0.1, 0.3, 1, 3, and 10 μM), with 5% fetal bovine serum (FBS), or with a combination of mevastatin and FBS. (B) Effect of mevalonic acid on mevastatin-induced inhibition of proliferation of theca–interstitial cells assessed by incorporation of radiolabeled thymidine; the cells were incubated for 48 hours without additives (control), with mevalonic acid (10, 30, and 100 μM), with mevastatin (3 μM), or with a combination of mevalonic acid and mevastatin. Each bar represents mean ± SEM (N = 8). *Means significantly different from control (P<.01). †Means significantly different from FBS (P<.01).
Kwintkiewicz. Proliferation of theca-interstitial cells. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

3. FIGURE 2
(A) Proliferation of theca–interstitial cells in the absence and in the presence of ovarian steroids; the cells were incubated for 48 hours without additives (control), with progesterone and testosterone, all at 1 and 10 nM. (B) Effect of insulin and mevastatin on proliferation of theca–interstitial cells assessed by incorporation of radiolabeled thymidine; the cells were incubated for 48 hours without additives (control), with insulin (30 nM), with mevastatin (3 μM), or with combination of insulin and mevastatin. Each bar represents mean ± SEM (N = 8), means with no superscripts in common are significantly different.
Kwintkiewicz. Proliferation of theca-interstitial cells. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

4. FIGURE 3
(A) Effect of insulin and mevastatin on phosphorylation of ERK1/2 (pTpY 185/187) of theca–interstitial cells. (B) Effect of insulin and mevastatin on phosphorylation of Akt/PKB (pS 473) of theca–interstitial cells. The cells were incubated for 10 minutes without additives (control), with insulin (100 nM), with mevastatin (30 μM), or with a combination of insulin and mevastatin. Each bar represents mean ± SEM (N = 5). Means with no superscript letters in common are significantly different (P<.05).
Kwintkiewicz. Proliferation of theca-interstitial cells. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions