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Pasquale Florio, M. D. , Ph. D. , Paulo B. Torres, M. D

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1 Human endometrium expresses urocortin II and III messenger RNA and peptides 
Pasquale Florio, M.D., Ph.D., Paulo B. Torres, M.D., Michela Torricelli, M.D., Paolo Toti, M.D., Wylie Vale, Ph.D., Felice Petraglia, M.D.  Fertility and Sterility  Volume 86, Issue 6, Pages (December 2006) DOI: /j.fertnstert Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

2 FIGURE 1 UCN II (A–E) and III (F–J) immunolocalization in human endometrium and pregnant decidua. (A) Proliferative endometrium. Intense UCN II immunostaining was mainly localized in epithelial luminal (black arrows) and glandular (white arrows) cells, whereas a weaker staining was found in stromal cells. Endothelial cells of the vessel walls (dotted circles) showed an intense staining. (B) Secretory endometrium. Epithelial glandular (white arrows) and luminal (black arrows) cells showed intense UCN II staining. Again, UCN II immunostaining was less intense in stromal cells. (C, D) Early pregnant endometrium. UCN II was localized in epithelial stromal (black arrows) and endothelial (dotted circles) cells from spiral arterioles. (E) Negative control obtained by using the antibody preadsorbed with UCN II (20 μg). (F) Proliferative endometrium. Intense UCN III immunostaining was mainly localized in epithelial luminal (black arrows) and glandular (white arrows) cells, whereas a weaker staining was found in stromal cells. Endothelial cells of the vessel walls (dotted circles) showed an intense staining. (G) Secretory endometrium. Epithelial glandular (white arrows) and luminal (black arrows) cells showed intense UCN III staining. Again, UCN III immunostaining was less intense in stromal cells. (H, I) Early pregnant endometrium. UCN III was localized in epithelial (black arrows), stromal, and endothelial (dotted circles) cells. (J) Negative control obtained by using the antibody preadsorbed with UCN III (20 μg). Florio. Urocortin 2 and 3 in human endometrium. Fertil Steril 2006. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

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