1. Surface biotinylation and fate of TREM2
Surface biotinylation and fate of TREM2 Surface‐expressed TREM2 on human macrophages was biotinylated at t = 0 and fractionated into four pools: supernatant, membrane‐associated, cytosolic and nuclear. Similar fractionation was undertaken following incubation for 0.5, 1.0 and 4.5 h. TREM2‐associated biotin was purified by immunoprecipitation and quantified by MSD for biotinylated TREM2. The raw and processed data are presented here. The left block of data shows biotin TREM2 measurements for four timepoints for four biological replicates for each of the four subcellular pools. The right block of data represents the normalised TREM2 quantification, with the membrane fraction at t = 0 defined as 100%.
Peter Thornton et al. EMBO Mol Med. 2017;emmm
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