1. Fully differentiated mucus-secreting human colon cancer HT29-MTX and HT29-FU cell subpopulations expressing the structural and functional characteristics of intestinal mucus-secreting goblet cells.
Fully differentiated mucus-secreting human colon cancer HT29-MTX and HT29-FU cell subpopulations expressing the structural and functional characteristics of intestinal mucus-secreting goblet cells. (A) Transmission electron microscopy examination of confluent fully differentiated HT29-MTX cells shows the polarized organization of the cells, the brush border, and the intracellular dense vesicles containing packaged mucins. (B) Transmission electron microscopy shows the intracellular vesicles containing packaged mucins near the apical domain. (C) Scanning electron microscopy examination shows the brush border of HT29-MTX cells after elimination of the mucus gel. (D) Scanning electron microscopy shows the secretion of mucus at the cell surface. (E and F) Scanning electron microscopy examination of HT29-MTX cells shows the dense mucus gel at the cell surface. (G) Confocal laser scanning microscopy examination shows the immunofluorescence labeling of secreted MUC5AC mucin in HT29-MTX cells (x-y section). (H) Scanning electron microscopy examination of postconfluent HT29-FU cells shows mucus secreted by a randomly distributed mucus-secreting HT29-FU cell and adjacent enterocyte-like HT29-FU cells. (I) High-magnification micrograph shows the dense and well-organized brush border in HT29-FU enterocyte-like cells. Confluent cells at 21 days in culture are shown. The arrows in A indicate the intracellular vesicles containing mucus.
Vanessa Liévin-Le Moal, and Alain L. Servin Microbiol. Mol. Biol. Rev. 2013; doi: /MMBR