1. Volume 16, Issue 6, Pages 612-617 (March 2006)
Reduced Heterozygosity Depresses Sperm Quality in Wild Rabbits, Oryctolagus cuniculus 
Matthew J.G. Gage, Alison K. Surridge, Joseph L. Tomkins, Emma Green, Louise Wiskin, Diana J. Bell, Godfrey M. Hewitt 
Current Biology 
Volume 16, Issue 6, Pages (March 2006)
DOI: /j.cub
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2. Figure 1
Across-Individual and Across-Population Relationships between Average Heterozygosity and Sperm Abnormality in Wild Rabbits
(A) The proportions of abnormal sperm (arcsine transformed) across 91 individual male rabbits show a significant negative relationship (R2 = 0.33 [±0.07], p < 0.001, Y = −0.58X ) with average heterozygosity. (Heterozygosity is scored across 29 genome-wide microsatellite loci and % sperm abnormalities scored from screens of 81 to 771 [average 320] sperm per male.) Removal of the three potential outliers preserves the relationship (R2 = 0.19, p < 0.001, n = 88). A similar relationship exists for % decapitated sperm, but not for the incidence of cytoplasmic droplets.
(B) Average proportions of abnormal sperm (arcsine transformed with standard errors) show a significant relationship with average heterozygosity across 12 sampling sites (least squares regression weighted by sample sizes: R2 = 0.7, p < 0.001; weighted by reciprocal of the variance: R2 = 0.58, p = ; removal of the outlier [represented by a single male]: R2 = 0.73, p = 0.001, n = 11). Large markers indicate means derived from >5 males, small markers <5 males. Sampling sites and actual sample sizes identified in Table S1.
Current Biology  , DOI: ( /j.cub )
Copyright © 2006 Elsevier Ltd Terms and Conditions

3. Figure 2
Across-Individual and Across-Population Relationships between Average Heterozygosity and Testis Size in Wild Rabbits
(A) Testis mass increases with heterozygosity across 112 males (R2 = 0.06 [±1.49], p = 0.008, Y = 3.52X ). Controlling for potential allometry in a multiple regression reveals a similar significant relationship between heterozygosity and testis weight/body weight combined (F2,109 = 3.78, p = 0.026; note that body mass does not correlate with heterozygosity: Rsp = 0.145, p = 0.13, n = 112).
(B) Average testis mass (with standard error bars) shows no relationship with mean heterozygosity across 12 populations (weighted means regression: R2 = 0.02 [±0.874], p = 0.2, n = 12). Large markers indicate means derived from >5 males, small markers <5 males. Sampling sites and actual sample sizes identified in Table S1.
Current Biology  , DOI: ( /j.cub )
Copyright © 2006 Elsevier Ltd Terms and Conditions