1. Ex. 18: Urea Hydrolysis (Urease Test)
Objectives ??

2. Work in a team of two students
Day 1
Materials needed per team of two students:
Urea agar slants (3)
Pure cultures needed per table:
Proteus vulgaris
Escherichia coli
Klebsiella pneumoniae
Work in a team of two students

3. urea This test checks if bacterium has enzyme urease
Medium contains urea, phenol red, peptones, glucose, potassium phosphate
Mainly used to differentiate Proteus from other Enterobacteriaceae

4. Table 18-1: Possible Results for Urease Test
Interpretation
Symbol
Up to 24 hours
24 hours to 6 days
All pink
Rapid urea hydrolysis; strong urease production +
Partially pink
All pink or partially pink
Slow urea hydrolysis; weak urease production
w +
Orange or yellow
No urea hydrolysis; urease is absent

5. the end