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Quantifying Instrumental Artifacts in Folding Kinetics Measured by Single-Molecule Force Spectroscopy  Krishna Neupane, Michael T. Woodside  Biophysical.

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Presentation on theme: "Quantifying Instrumental Artifacts in Folding Kinetics Measured by Single-Molecule Force Spectroscopy  Krishna Neupane, Michael T. Woodside  Biophysical."— Presentation transcript:

1 Quantifying Instrumental Artifacts in Folding Kinetics Measured by Single-Molecule Force Spectroscopy  Krishna Neupane, Michael T. Woodside  Biophysical Journal  Volume 111, Issue 2, Pages (July 2016) DOI: /j.bpj Copyright © 2016 Biophysical Society Terms and Conditions

2 Figure 1 Analysis of rates. (A) Extension trajectory for hairpin 30R50/T4 at constant force. (Upper inset) Schematic of measurement showing hairpin attached to beads via DNA handles. (Right inset) Probability density from trajectory. (B) Decay of extension autocorrelation in unfolded state yields τA. (C) PMF from the extension trajectory, yielding κAb, κAw, and ΔGA‡. (D) Landscape obtained by deconvolution of PMF. To see this figure in color, go online. Biophysical Journal  , DOI: ( /j.bpj ) Copyright © 2016 Biophysical Society Terms and Conditions

3 Figure 2 Constant trap position measurements. (A) Extension trajectory for hairpin 20TS06/T4. (B) Decay of extension autocorrelation in unfolded state yields τA. (C) PMF from extension trajectory yields κAb, κAw, and ΔGA‡. To see this figure in color, go online. Biophysical Journal  , DOI: ( /j.bpj ) Copyright © 2016 Biophysical Society Terms and Conditions

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