1. Stress Response, Tachykinin, and Cutaneous Inflammation
Ichiro Katayama, Sang-Jae Bae, Yo-ichiro Hamasaki 
Journal of Investigative Dermatology Symposium Proceedings 
Volume 6, Issue 1, Pages (November 2001)
DOI: /j x x
Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

2. Figure 1
Experimental protocol. BALB/C mice received nine epicutaneous applications of 0.1% diflucotolone on alternate days on the flank skin. After seven applications, each mouse was sensitized with DNFB, TNCB. or oxazolone. Twenty-four hours after the last applications of 0.1% diflucotolone, each mouse was challenged with corresponding hapten on the right pinna skin. The left pinna was used as vehicle control.
Journal of Investigative Dermatology Symposium Proceedings 2001 6, 81-86DOI: ( /j x x)
Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

3. Figure 2
Augmentation of contact sensitivity skin reaction by GC.Igawa et al (1997). 0.1% diflucotolone valerate was applied on the flank skin nine times on alternate days (seven applications before and two applications after sensitization). Mice were sensitized with 0.5% DNFB on day 12 (seventh application of diflucotolone valerate) on the same site of flank skin that mice received 0.1% diflucotolone seven times, and then skin was tested on the right pinna on day 17 (one day after the nine applications of diflucotolone valerate). (A) Dose response of diflucotolone valerate on DNFB contact sensitivity reaction; (B) effect of a different kind of GC on DNFB contact sensitivity reaction.
Journal of Investigative Dermatology Symposium Proceedings 2001 6, 81-86DOI: ( /j x x)
Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

4. Figure 3
Augmentation of IgE anti-DNP antibody induced passive cutaneous anaphylaxis elicited on the pinna by long-term topical GC applied on distant flank skin. Fifty microliters of 0.1% diflucortolone valerate or 1α-24 (OH)2 D3 (0.05 µg per ml) in ethanol were applied on the flank skin seven times on alternate days. Mice then received intravenous application of monoclonal IgE anti-DNP antibody 1h after the last application (final PCA titer > ×2560). One hour after injection, mice were challenged with 0.15% DNFB in acetone:olive oil (4:1) on the right pinna. The left pinna was painted with vehicle as a control.
Journal of Investigative Dermatology Symposium Proceedings 2001 6, 81-86DOI: ( /j x x)
Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

5. Figure 4
Effect of hydrocortisone on IL1α production by Pam 212 cells through NFĸB activation.Miyazaki et al (2000). (A) Various concentrations of hydrocortisone were added to the culture medium of Pam 212 KC cell lines and IL1α concentration was measured by ELISA 24h after stimulation. (B) Effect of PDTC on TNBS and hydrocortisone-induced NFĸB expression and IL1α production by Pam 212 cells.
Journal of Investigative Dermatology Symposium Proceedings 2001 6, 81-86DOI: ( /j x x)
Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

6. Figure 5
Time course and concentration-dependent production of SP by cultured KC.Bae et al (1999). Normal human KC were cultured in KGM CC-3111 medium (complete medium) supplemented with BPE, hEGF, insulin, antibiotics, and hydrocortisone as described above. At the semiconfluent stage, the cells were washed with ice-chilled DMEM and replaced with KGM medium without hydocortisone, for 24h. At the start of the experiment, the medium was replaced with fresh KGM medium containing variously diluted SP and then culture supernatants were collected at various times and donated for ELISA assay. (A) The dose–response effect of SP on autocrine production of SP in cultured normal human KC (NHK). (B) Time course of SP-induced SP production in cultured normal human KC (NHK). Results are expressed as the means ± SD.
Journal of Investigative Dermatology Symposium Proceedings 2001 6, 81-86DOI: ( /j x x)
Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

7. Figure 6
SP gene expression in SP-stimulated normal KC.Bae et al (1999). To clarify the SP gene expression in normal KC, we performed RT-PCR and Southern blot analysis. For specificity of mRNA expression of PPT-A, we performed Southern blot analysis as described in the Materials and Methods. The values were determined by scanning densitometer and expressed in relation to actin scanning density.
Journal of Investigative Dermatology Symposium Proceedings 2001 6, 81-86DOI: ( /j x x)
Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

8. Figure 7
Stress-induced augmentation of cutaneous inflammation. Stress-induced GC or long-term topical GC and its withdrawal might induce epidermal cytokine and SP, resulting in augmented cutaneous inflammations. Th1-derived IFNγ or Th1/Th2-derived IL-3 might modulate these circuits.
Journal of Investigative Dermatology Symposium Proceedings 2001 6, 81-86DOI: ( /j x x)
Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions