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Valve interstitial cells induce donor-specific T-cell anergy

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Presentation on theme: "Valve interstitial cells induce donor-specific T-cell anergy"— Presentation transcript:

1 Valve interstitial cells induce donor-specific T-cell anergy
Puspa Batten, PhD, Ann M. McCormack, BSc, Marlene L. Rose, PhD, Magdi H. Yacoub, FRS  The Journal of Thoracic and Cardiovascular Surgery  Volume 122, Issue 1, Pages (July 2001) DOI: /mtc Copyright © 2001 American Association for Thoracic Surgery Terms and Conditions

2 Fig. 1 Purified human CD4+ T cells but not CD8+ T cells proliferate to valve endothelial cells. Data are expressed as mean counts per minute ± SD of triplicate wells for T-cell proliferation. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /mtc ) Copyright © 2001 American Association for Thoracic Surgery Terms and Conditions

3 Fig. 2 Valve interstitial cells do not elicit CD4+ T-cell proliferation. Data are expressed as mean counts per minute ± SD of trip-licate wells for T-cell proliferation. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /mtc ) Copyright © 2001 American Association for Thoracic Surgery Terms and Conditions

4 Fig. 3 Phenotypic characterization of endothelial and interstitial cells cultured from valve and nonvalve tissue. Expression of cell-surface antigens before and after IFN-γ treatment was carried out with appropriate mAbs against HLA class I, HLA class II, CD40, lymphocyte function-associated antigen 3 (LFA-3), and intercellular adhesion molecule 1 (ICAM-1), as described in the “Materials and Methods” section. The negative controls were fluorescein isothiocyanate-conjugated antimouse secondary antibodies only. Data are expressed as percentages of positive staining. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /mtc ) Copyright © 2001 American Association for Thoracic Surgery Terms and Conditions

5 Fig. 4 Valve interstitial cells induce a state of T-cell nonresponsiveness. A, CD4+ T cells respond to both IFN-γ–treated mitral valve endothelial cells 58 (MV58 EC) and to third-party tricuspid valve endothelial cells (TV71 EC) for 6 days. B, The 2-step tolerance induction protocol described in the “Materials and Methods” section was used. Data are expressed as mean counts per minute ± SD of triplicate wells. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /mtc ) Copyright © 2001 American Association for Thoracic Surgery Terms and Conditions

6 Fig. 5 T-cell responses to valve interstitial cells are rescued by B7-1 transcostimulation. CD4+ T cells (105) were cocultured with 2.5 × 104 untreated or IFN-γ–treated TV84 endothelial cells (EC) or interstitial cells (IC). The transfectant, DAP.B7, which expresses human B7-1, was also added to either T-cell responses to IFN-γ–treated TV84 interstitial cells or to T cells alone. Data are expressed as mean counts per minute ± SD of triplicate wells. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /mtc ) Copyright © 2001 American Association for Thoracic Surgery Terms and Conditions

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