Presentation is loading. Please wait.

Presentation is loading. Please wait.

Effects of established allergen sensitization on immune and airway responses after secondary allergen sensitization  Katharina Blumchen, MD, Kerstin Gerhold,

Published byÂngelo Ximenes Modified over 5 years ago

Similar presentations

Presentation on theme: "Effects of established allergen sensitization on immune and airway responses after secondary allergen sensitization  Katharina Blumchen, MD, Kerstin Gerhold,"— Presentation transcript:

1 Effects of established allergen sensitization on immune and airway responses after secondary allergen sensitization  Katharina Blumchen, MD, Kerstin Gerhold, MD, Marcus Schwede, MD, Bodo Niggemann, MD, Anzhela Avagyan, MD, Anna-Maria Dittrich, MD, Birgit Wagner, PhD, Heimo Breiteneder, PhD, Eckard Hamelmann, MD  Journal of Allergy and Clinical Immunology  Volume 118, Issue 3, Pages (September 2006) DOI: /j.jaci Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

2 Fig 1 Experimental protocol. Mice were immunized with either ovalbumin or latex as a primary sensitization followed by a secondary systemic sensitization with the other allergen (OVA/Latex, Latex/OVA). Single-sensitized mice received a sham sensitization followed by sensitization with either latex (PBS/Latex) or ovalbumin (PBS/OVA). A control group was purely sham-sensitized (PBS/PBS). All animals received airway challenges with the second allergen. in, Intranasal; ip, intraperitoneal; Neb, via nebulization. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

3 Fig 2 Total IgE. Experimental protocol as indicated in Fig 1. Mice were bled on day 72, and serum total IgE was determined by ELISA. Double-sensitized vs single-sensitized mice: PBS/Latex vs OVA/Latex, PBS/OVA vs Latex/OVA, ∗∗P < .01, ∗P < .05. Sham-sensitized vs allergen-sensitized mice: PBS/PBS vs PBS/Latex or PBS/OVA, ++P < .01. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

4 Fig 3 Mitogen-induced cytokine production by spleen MNCs. Experimental protocol as indicated in Fig 1. Splenic MNCs were obtained on day 72 and restimulated in vitro with concanavalin A. Cytokine production in supernatants was measured by ELISA: IL-4 (A), IL-5 (B), and IFN-γ (C). Single-sensitized vs double-sensitized mice: PBS/Latex vs OVA/Latex, PBS/OVA vs Latex/OVA, ∗∗P < .01, ∗P < .05. Sham-sensitized vs allergen-sensitized mice: PBS/PBS vs PBS/Latex or PBS/OVA, ++P < .01. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

5 Fig 4 Latex-specific immunoglobulin production. Experimental protocol as indicated in Fig 1. Mice were bled on day 72. Latex-specific IgE (A), IgG1 (B), and IgG2a (C) were evaluated by ELISA in measuring immunoglobulin production specific for Hev b 3, a major latex allergen. Levels are documented in OD units. Single-sensitized vs double-sensitized mice: PBS/Latex vs OVA/Latex, ∗P < .05. Sham-sensitized vs single-sensitized mice: PBS/PBS vs PBS/Latex, ++P < .01, +P < .05. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

6 Fig 5 Ovalbumin-specific immunoglobulin production. Experimental protocol as indicated in Fig 1. Mice were bled on day 72. Ovalbumin-specific IgE (A), IgG1 (B), and IgG2a (C) were measured by ELISA and compared with known hyperimmune standards (EU/mL). Single-sensitized vs double-sensitized mice: PBS/OVA vs Latex/OVA, ∗∗P < .01. Purely sham-sensitized animals (PBS/PBS) showed OVA-IgE, IgG1, and IgG2a below detection limits. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

7 Fig 6 Bronchoalveolar lavage. Experimental design as indicated in Fig 1. On day 72, two days after last airway challenge, lungs were lavaged, and differential cell counts in bronchoalveolar lavage fluids were obtained. Total cell counts, eosinophil counts, and lymphocyte counts are presented. Single-sensitized vs double-sensitized mice: PBS/Latex vs OVA/Latex, ∗P < .05. Sham-sensitized vs allergen-sensitized mice: PBS/PBS vs PBS/Latex or OVA/Latex, ++P < .01. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

8 Fig 7 Airway reactivity. Experimental design as indicated in Fig 1. On day 71, lung function to inhaled methacholine was measured by whole-body plethysmography. The dosage of methacholine increasing AR by 200% (PC 200) or 500% (PC 500) was calculated for each animal. A, AR of mice that received OVA as primary and Latex as secondary sensitization agent (OVA/Latex). B, AR of mice that received the reversed protocol (Latex/OVA). Single-sensitized vs double-sensitized mice: PBS/Latex vs OVA/Latex, PBS/OVA vs Latex/OVA, ∗∗P < .01, ∗P < .05. Sham-sensitized vs allergen-sensitized mice: PBS/PBS vs PBS/Latex, PBS/OVA or double-sensitized mice, +P < .05. MCh, Methacholine. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2006 American Academy of Allergy, Asthma and Immunology Terms and Conditions

Download ppt "Effects of established allergen sensitization on immune and airway responses after secondary allergen sensitization  Katharina Blumchen, MD, Kerstin Gerhold,"

Similar presentations

Dual effects of vitamin D–induced alteration of TH1/TH2 cytokine expression  Victor Matheu, MD, Ove Bäck, MD, PhD, Emma Mondoc, BSc, Shohreh Issazadeh-Navikas,

Dual effects of vitamin D–induced alteration of TH1/TH2 cytokine expression  Victor Matheu, MD, Ove Bäck, MD, PhD, Emma Mondoc, BSc, Shohreh Issazadeh-Navikas,
Exposure to allergen and diesel exhaust particles potentiates secondary allergen- specific memory responses, promoting asthma susceptibility  Eric B. Brandt,

Exposure to allergen and diesel exhaust particles potentiates secondary allergen- specific memory responses, promoting asthma susceptibility  Eric B. Brandt,
Effective prevention and therapy of experimental allergic asthma using a GATA-3– specific DNAzyme  Serdar Sel, MD, Michael Wegmann, PhD, Tanja Dicke, MSc,

Effective prevention and therapy of experimental allergic asthma using a GATA-3– specific DNAzyme  Serdar Sel, MD, Michael Wegmann, PhD, Tanja Dicke, MSc,
The activating protein 1 transcription factor basic leucine zipper transcription factor, ATF- like (BATF), regulates lymphocyte- and mast cell–driven immune.

The activating protein 1 transcription factor basic leucine zipper transcription factor, ATF- like (BATF), regulates lymphocyte- and mast cell–driven immune.
The activating protein 1 transcription factor basic leucine zipper transcription factor, ATF- like (BATF), regulates lymphocyte- and mast cell–driven immune.

The activating protein 1 transcription factor basic leucine zipper transcription factor, ATF- like (BATF), regulates lymphocyte- and mast cell–driven immune.
Designing hypoallergenic derivatives for allergy treatment by means of in silico mutation and screening  Theresa Thalhamer, PhD, Heidi Dobias, MSc, Tatjana.

Designing hypoallergenic derivatives for allergy treatment by means of in silico mutation and screening  Theresa Thalhamer, PhD, Heidi Dobias, MSc, Tatjana.
Prior airway exposure to allergen increases virus-induced airway hyperresponsiveness  Mika J Mäkelä, MD, Ralph Tripp, PhD, Azzeddine Dakhama, PhD, Jun-Won.

Prior airway exposure to allergen increases virus-induced airway hyperresponsiveness  Mika J Mäkelä, MD, Ralph Tripp, PhD, Azzeddine Dakhama, PhD, Jun-Won.
Virus-specific IgE enhances airway responsiveness on reinfection with respiratory syncytial virus in newborn mice  Azzeddine Dakhama, PhD, Young-Mok Lee,

Virus-specific IgE enhances airway responsiveness on reinfection with respiratory syncytial virus in newborn mice  Azzeddine Dakhama, PhD, Young-Mok Lee,
Allergy prevention starts before conception: Maternofetal transfer of tolerance protects against the development of asthma  Tobias Polte, PhD, Christian.

Allergy prevention starts before conception: Maternofetal transfer of tolerance protects against the development of asthma  Tobias Polte, PhD, Christian.
Interferon response factor 3 is essential for house dust mite–induced airway allergy  Thomas Marichal, DVM, Denis Bedoret, DVM, PhD, Claire Mesnil, DVM,

Interferon response factor 3 is essential for house dust mite–induced airway allergy  Thomas Marichal, DVM, Denis Bedoret, DVM, PhD, Claire Mesnil, DVM,
Selective control of SIRP-α–positive airway dendritic cell trafficking through CD47 is critical for the development of TH2-mediated allergic inflammation 

Selective control of SIRP-α–positive airway dendritic cell trafficking through CD47 is critical for the development of TH2-mediated allergic inflammation 
Expression of IL-4 receptor α on smooth muscle cells is not necessary for development of experimental allergic asthma  Frank Kirstein, PhD, William G.C.

Expression of IL-4 receptor α on smooth muscle cells is not necessary for development of experimental allergic asthma  Frank Kirstein, PhD, William G.C.
Frank Kirstein, PhD, Natalie E

Frank Kirstein, PhD, Natalie E
Maternal house dust mite exposure during pregnancy enhances severity of house dust mite–induced asthma in murine offspring  Phoebe K. Richgels, MS, Amnah.

Maternal house dust mite exposure during pregnancy enhances severity of house dust mite–induced asthma in murine offspring  Phoebe K. Richgels, MS, Amnah.
Protective role of nuclear factor of activated T cells 2 in CD8+ long-lived memory T cells in an allergy model  Roman Karwot, PhD, Joachim H. Maxeiner,

Protective role of nuclear factor of activated T cells 2 in CD8+ long-lived memory T cells in an allergy model  Roman Karwot, PhD, Joachim H. Maxeiner,
Elisabeth Roesler, MSc, Richard Weiss, PhD, Esther E

Elisabeth Roesler, MSc, Richard Weiss, PhD, Esther E
Exposure to allergen and diesel exhaust particles potentiates secondary allergen- specific memory responses, promoting asthma susceptibility  Eric B. Brandt,

Exposure to allergen and diesel exhaust particles potentiates secondary allergen- specific memory responses, promoting asthma susceptibility  Eric B. Brandt,
Protein corona–mediated targeting of nanocarriers to B cells allows redirection of allergic immune responses  Limei Shen, PhD, Stefan Tenzer, PhD, Wiebke.

Protein corona–mediated targeting of nanocarriers to B cells allows redirection of allergic immune responses  Limei Shen, PhD, Stefan Tenzer, PhD, Wiebke.
IL-33 dysregulates regulatory T cells and impairs established immunologic tolerance in the lungs  Chien-Chang Chen, PhD, Takao Kobayashi, PhD, Koji Iijima,

IL-33 dysregulates regulatory T cells and impairs established immunologic tolerance in the lungs  Chien-Chang Chen, PhD, Takao Kobayashi, PhD, Koji Iijima,
Airway inflammation after epicutaneous sensitization of mice requires protease activity of low-dose allergen inhalation  Izumi Nishioka, MD, PhD, Toshiro.

Airway inflammation after epicutaneous sensitization of mice requires protease activity of low-dose allergen inhalation  Izumi Nishioka, MD, PhD, Toshiro.

Similar presentations

Ads by Google