1. 1
Oncomine database: DKK2 upregulation in colorectal cancers(CRC)
APC mutant mice: intestinal tumor model DKK2 expression
TCGA CRC data set: high DKK2  poor survival
DKK2-antibody: 5F8 (prevent binding to LRP5) enhances NK/CD8, activation
DKK2-/- increased apoptosis, GZMB production by NK & CD8+
5F8: 24 h treatment 2 3
In vitro system:
NK/IL15+ MC 38 cells coculture 5F8 treatment or recombinant DKK2
Viability, GZMB, gene analyses 4
DKK2-related signaling: Wnt--catenin,
RNA-seq analysis STAT5
DKK2 bind to LRP5 (internization); MC38 tumor growth slow in LRP5-/- mice
LRP5/STAT5 co-IP, (prevent nuclear localization of STAT5)
LRP5C inhibited STAT5 reporter gene 5 6
DKK2 and anti-PD-1