1. miR‐146b induces 3T3‐L1 adipocyte differentiation qRT‐PCR analysis of miR‐146b during 3T3‐L1 differentiation (n = 4).
miR‐146b induces 3T3‐L1 adipocyte differentiation qRT‐PCR analysis of miR‐146b during 3T3‐L1 differentiation (n = 4). Values are means ± SD. *p < 0.05 compared with day 0.The effect of miR‐146b activator (Ac) on miR‐146b expression. Undifferentiated 3T3‐L1 cells were transfected with miR‐146 Ac or miR Ac control (CTL) and maintained in DMEM supplemented with 10% FBS for 8 d. Expression of miR‐146b was calculated relative to MOCK (n = 3). Values are means ± SD. *p < 0.05 versus miR Ac CTL.The effect of miR‐146b Ac on intracellular lipid accumulation in undifferentiated 3T3‐L1 cells. Lipid droplets were detected by Oil red O staining.Intracellular lipid accumulation was quantified by measuring optical absorbance at 500 nm (n = 3). Values are means ± SD. *p < 0.05 versus miR Ac CTL.Western blotting was performed for various adipocyte differentiation markers.The effect of miR‐146b inhibitor (In) on miR‐146b expression. 3T3‐L1 cells transfected with miR‐146b or miR In CTL were differentiated according to a standard protocol. Expression of miR‐146b was calculated relative to MOCK (n = 3). Values are means ± SD. *p < 0.05 versus miR In CTL.The effect of miR‐146b In on intracellular lipid accumulation in differentiated 3T3‐L1 cells. Intracellular lipid accumulation was stained with Oil red O and quantitated by spectrophotometric measurement (n = 3). Values are means ± SD. *p < 0.05 versus miR In CTL.Western blotting was performed for various adipocyte differentiation markers.
Jiyun Ahn et al. EMBO Mol Med. 2013;5:
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