1. Effects of photodynamic therapy using 5-aminolevulinic acid on cultured human adenomyosis-derived cells 
Haruka Suzuki-Kakisaka, M.D., Takashi Murakami, M.D., Ph.D., Toru Hirano, Ph.D., Yukihiro Terada, M.D., Ph.D., Nobuo Yaegashi, M.D., Ph.D., Kunihiro Okamura, M.D., Ph.D. 
Fertility and Sterility 
Volume 87, Issue 1, Pages (January 2007)
DOI: /j.fertnstert
Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions

2. FIGURE 1
Adenomyosis-derived cell cultures. (A) Original tissure stained with H&E; the asterisk indicates adenomyosis lesion. (Original magnification: ×200.) (B) Confluent cultured cells shown by phase-contrast (Original magnification: ×100). (C–H) Immunocytochemical characterization of stromal cells derived from adenomyosis tissue. Cultured cells stained by vimentin (C), but not by cytokeratin (D), vWF (E), or α-smooth muscle actin (G). Uterine smooth muscle was stained with smooth muscle actin (H). (F) Negative control. (—: 10 μm.)
Suzuki-Kakisaka. Effects of a PDT on adenoymosis. Fertil Steril 2007.
Fertility and Sterility  , 33-38DOI: ( /j.fertnstert )
Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions

3. FIGURE 2
Increased cell death with prolonged ALA pretreatment time. ALA-induced phototoxicity in cells derived from adenomyosis tissue (A) or endometrium (B) exposed to 100 μg/mL of ALA for the indicated periods of time followed by the indicated radiation doses. Cell death was dependent upon both incubation time and radiation dose.
Suzuki-Kakisaka. Effects of a PDT on adenoymosis. Fertil Steril 2007.
Fertility and Sterility  , 33-38DOI: ( /j.fertnstert )
Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions

4. FIGURE 3
The % cell survival rates by MTT assay at the point of 48 hours from Figure 2 are shown. (A) The survival rate following PDT with 5-ALA of the stromal cells derived from adenomyosis or (B) endometrium. (A) ALA only, (B) PDT (1.6 J/cm2), (C) PDT (4 J/cm2), (D) PDT (8 J/cm2). In (A), PDT using >4 J/cm2 are significant effective for the suppression of cells compared with ALA only. Data are the mean SD (n = 4). *P<.05 compared to ALA-alone treated cells.
Suzuki-Kakisaka. Effects of a PDT on adenoymosis. Fertil Steril 2007.
Fertility and Sterility  , 33-38DOI: ( /j.fertnstert )
Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions