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Thromboxane-Induced Contractile Response of Human Coronary Arterioles Is Diminished After Cardioplegic Arrest  Jun Feng, MD, PhD, Yuhong Liu, MD, Louis.

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Presentation on theme: "Thromboxane-Induced Contractile Response of Human Coronary Arterioles Is Diminished After Cardioplegic Arrest  Jun Feng, MD, PhD, Yuhong Liu, MD, Louis."— Presentation transcript:

1 Thromboxane-Induced Contractile Response of Human Coronary Arterioles Is Diminished After Cardioplegic Arrest  Jun Feng, MD, PhD, Yuhong Liu, MD, Louis M. Chu, MD, Richard T. Clements, PhD, Kamal R. Khabbaz, MD, Michael P. Robich, MD, Cesario Bianchi, MD, PhD, Frank W. Sellke, MD  The Annals of Thoracic Surgery  Volume 92, Issue 3, Pages (September 2011) DOI: /j.athoracsur Copyright © 2011 The Society of Thoracic Surgeons Terms and Conditions

2 Fig 1 (A) Coronary arteriolar vasoconstriction in response to the thromboxane A (TXA)-2 analog U before vs after cardioplegic ischemia, followed by reperfusion (pre-CP/Rep [white circles] vs post-CP/Rep [black circles]); *p < 0.05 vs pre-CP/Rep. (B) Administration of U with or without TXA-2 antagonist SQ to microvessels of pre-CP/Rep (U [pre-CP/Rep, white circles] or SQ U [pre-CP/Rep, black squares]); *p < 0.05 vs U (pre-CP/Rep). (C) Infusion of U-46619, with or without the TXA-2 antagonist SQ-29548, to post-CP/Rep vessels (U [post-CP/Rep, black circles] or SQ U [post-CP/Rep, black diamonds]); p < 0.05 vs U (post-CP/Rep); n = 6 to 8/group. The bars show the standard error of the mean. The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur ) Copyright © 2011 The Society of Thoracic Surgeons Terms and Conditions

3 Fig 2 (A) Administration of U to coronary arteriole before cardioplegic ischemia, followed by reperfusion (pre-CP/Rep), with or without the presence of the phospholipase-C (PLC) inhibitor U (U [Pre-CP/Rep, white circles] or U U [pre-CP/Rep, white triangles]), *p < 0.05 vs U (pre-CP/Rep). (B) Perfusion of U to post-CP/Rep coronary arterioles, with or without the presence of the PLC inhibitor U (U [post-CP/Rep, black circles] or U U [post-CP/Rep, black squares]),*p < 0.05 vs. U (post-CP/Rep); n = 6 to 8/group. The bars show the standard error of the mean. The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur ) Copyright © 2011 The Society of Thoracic Surgeons Terms and Conditions

4 Fig 3 (A) Addition of U to the vessels from samples before cardioplegic ischemia, followed by reperfusion (pre-CP/Rep), in the absence or presence of the protein kinase C (PKC) inhibitor safingol (U [pre-CP/Rep, white circles] or safingol + U [pre-CP/Rep, white diamonds]). (B) Addition of U to the vessels from post-CP/Rep samples in the absence or presence of PKC inhibitor safingol (U [post-CP/Rep, black circles] or safingol + U [post-CP/Rep, black diamonds]); n= 6 to 8/group. The bars show the standard error of the mean. The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur ) Copyright © 2011 The Society of Thoracic Surgeons Terms and Conditions

5 Fig 4 Representative immunoblot of human atria tissue. Lanes 1 to 12, loaded with 40 μg protein, were developed for (A, top) thromboxane A (TXA)-2 receptor and TXA-2 synthase (n = 6 ) and (B, top) phospholipase-C (PLC)-β3, phospho–PLC-β3, PLC-γ1, and phosphor–PLC-γ1 polypeptides (n = 6). Immunoblot band intensity shows unaltered levels of TXA-2 receptor, TXA-2 synthase, PLC-γ1, and PLC-β3 polypeptides after cardioplegic ischemia, followed by reperfusion (CP/Rep; bottom of Fig 4A and B), respectively. The bars show the standard error of the mean. The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur ) Copyright © 2011 The Society of Thoracic Surgeons Terms and Conditions

6 Fig 5 Immunolocalization of thromboxane A (TXA)-2 receptor, phospholipase-C (PLC)-γ1, and PLC-β3 polypeptides in human coronary microvessels (n = 6). Vessels were costained for smooth muscle α-actin with (A) TXA-2 receptor antibody or (B) PLC-β3. Matched negative controls are displayed below each row of primary antibody. (DAPI = 4',6-diamidino-2-phenylindole.) The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur ) Copyright © 2011 The Society of Thoracic Surgeons Terms and Conditions

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