1. Technical optimization of oral gavage technique.
Technical optimization of oral gavage technique. (A) The gavage apparatus was constructed from a 10 µl luer-tip Hamilton syringe with a 22 G needle and 22 G soft-tip catheter tubing. (B) A representative zebrafish was anesthetized using a MS-222/isoflurane combination anesthetic and immobilized vertically in a damp sponge with a slit. (C) A representative zebrafish was gavaged with 3 μl fluorescein isothiocyanate-dextran (FITC-dextran) to visualize potential regurgitation via fluorescence microscopy. (D) Zebrafish were gavaged with Phenol Red solution to visualize potential regurgitation. Data represented as mean±s.d. of two replicates. (E) Survival curve for extended exposure to anesthetic solutions. Data represented as mean±s.d. of three replicates. (F) Survival curve for long-term daily exposure to anesthetic solution. Data represented as mean±s.d. of three replicates.
Michelle Dang et al. Dis. Model. Mech. 2016;9:
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