1. Macrophage migration inhibitory factor expression in the intrauterine endometrium of women with endometriosis varies with disease stage, infertility status, and pelvic pain 
Ali Akoum, Ph.D., Christine N. Metz, Ph.D., Mahera Al-Akoum, M.D., Rouslan Kats, M.Sc. 
Fertility and Sterility 
Volume 85, Issue 5, Pages (May 2006)
DOI: /j.fertnstert
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

2. FIGURE 1
Immunohistochemical detection of MIF in the endometrium. Tissue sections were successively incubated with a goat polyclonal anti-MIF antibody, a biotin-conjugated rabbit antigoat antibody, streptavidin-peroxidase, DAB, and counterstained with hematoxylin (blue staining). (A, C) Tissue from women with endometriosis in the proliferative (day 9 of 28) and the secretory (day 24 of 28) phases of the cycle, respectively. (B, D) Tissue from normal women in the proliferative (day 10 of 28) and the secretory (day 26 of 28) phases of the cycle, respectively. Note positive brown immunostaining in the glands (g) and cell aggregates (c) scattered throughout the stroma, which was more intense in women with endometriosis as compared with normal subjects, particularly in the secretory phase of the menstrual cycle. (E) Representative photomicrograph showing no staining in a serial section from the endometrial tissue shown in C, incubated with normal mouse immunoglobulins instead of the primary antibody taken as negative control. Scale bar = 100 μm.
Akoum. Endometrial MIF expression in endometriosis. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

3. FIGURE 2
Distribution of MIF concentrations in the endometrial tissue of normal controls (n = 25) and patients with endometriosis (n = 45), according to disease stage. (A) Controls (N) vs. women with endometriosis (E). (B) Controls vs. women with endometriosis stages I, II, and III. Horizontal lines represent the mean for each set of data. *P<.05; ***P<.001; ****P<.0001 vs. controls.
Akoum. Endometrial MIF expression in endometriosis. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

4. FIGURE 3
Distribution of MIF concentrations in the endometrial tissue of normal controls (n = 25) and patients with endometriosis (n = 45), according to (A) fertility status and (B) pelvic pain. Horizontal lines represent the mean for each set of data. *P<.05; **P<.01; ***P<.001 vs. controls.
Akoum. Endometrial MIF expression in endometriosis. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

5. FIGURE 4
Distribution of MIF concentrations in the endometrial tissue of normal controls (n = 25) and patients with endometriosis (n = 45), according to menstrual cycle phase. (A) Proliferative (P) and secretory (S) phases. (B) Early to mid-proliferative phase, days 1–9 (1), late proliferative to early secretory phase, days 10–18 (2), midsecretory phase, days 19–23 (3), and late secretory phase, days 24–28 (4). Horizontal lines represent the mean for each set of data. *P<.05; **P<.01; ***P<.001 vs. controls.
Akoum. Endometrial MIF expression in endometriosis. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions