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Effect of a glucosamine derivative on impact-induced chondrocyte apoptosis in vitro. A preliminary report  C.A.M. Huser, Ph.D., M.E. Davies, Ph.D.  Osteoarthritis.

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Presentation on theme: "Effect of a glucosamine derivative on impact-induced chondrocyte apoptosis in vitro. A preliminary report  C.A.M. Huser, Ph.D., M.E. Davies, Ph.D.  Osteoarthritis."— Presentation transcript:

1 Effect of a glucosamine derivative on impact-induced chondrocyte apoptosis in vitro. A preliminary report  C.A.M. Huser, Ph.D., M.E. Davies, Ph.D.  Osteoarthritis and Cartilage  Volume 16, Issue 1, Pages (January 2008) DOI: /j.joca Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

2 Fig. 1 Effect of a single impact load and pre-incubation with (A) N-Glu and Glu5 and (B) N-Glu and Glu11 on the percentage TUNEL-positive chondrocytes in the superficial zone of cartilage explants after 48h in culture. Bars represent mean±standard deviation (SD). # Represents a significant difference from 0mm unimpacted control and * represents a significant difference from 50mm impacted untreated control treated with DMSO vehicle only (analysis of variance-2 [ANOVA-2], P<0.05). Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

3 Fig. 2 Effect of a single impact load and pre-incubation with culture medium containing Glu5 on the percentage of superficial chondrocytes with a JC-1 ratio larger than 0.9, indicating mitochondrial depolarisation, after 6h in culture. Bars represent mean±SD. # Represents a significant difference from 0mm unimpacted control (Mann–Whitney, P<0.05). Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions


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