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The Distribution of Gene Segments in T-Cell Receptor γ Gene Rearrangements Demonstrates the Need for Multiple Primer Sets  Lyle C. Lawnicki, Ronald J.

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Presentation on theme: "The Distribution of Gene Segments in T-Cell Receptor γ Gene Rearrangements Demonstrates the Need for Multiple Primer Sets  Lyle C. Lawnicki, Ronald J."— Presentation transcript:

1 The Distribution of Gene Segments in T-Cell Receptor γ Gene Rearrangements Demonstrates the Need for Multiple Primer Sets  Lyle C. Lawnicki, Ronald J. Rubocki, Wing C. Chan, Deborah M. Lytle, Timothy C. Greiner  The Journal of Molecular Diagnostics  Volume 5, Issue 2, Pages (May 2003) DOI: /S (10) Copyright © 2003 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

2 Figure 1 Denaturing gradient gel electrophoresis: two cases exhibiting a rearrangement in the Vγ10 segment (arrows). CEM, HSB2, and Jurkat are T-cell lines for positive controls and CEM shows a biallelic rearrangement. The Journal of Molecular Diagnostics 2003 5, 82-87DOI: ( /S (10) ) Copyright © 2003 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

3 Figure 2 Capillary electrophoresis method showing biallelic clonal rearrangements with the Jγ1/2 (black) and JγP1/2 (green) primers in a case of peripheral T-cell lymphoma. The heights of the clonal peaks are greater than two times the polyclonal background. x axis, nucleotide length; y axis, intensity of signal. The Journal of Molecular Diagnostics 2003 5, 82-87DOI: ( /S (10) ) Copyright © 2003 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

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