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Presence of pink pigment + / -

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Presentation on theme: "Presence of pink pigment + / -"— Presentation transcript:

1 Presence of pink pigment + / -
Four-o’clock plants Incomplete dominance the term used to describe the general case in which the phenotype of a heterozygote is intermediate between those of the two homozygotes, on some quantitative scale of measurement

2 Point mutations at the molecular level
Purine replaced by a different purine; pyrimidine replaced by a different pyrimidine: TRANSITIONS Purine replaced by a pyrimidine; pyrimidine replaced by a purin: TRANSVERSIONS

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10 Now, insertions and deletions of base pairs:

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12 Selection of auxotrophs by filter enrichment
Forward mutation-A mutation that converts a wild-type allele into a mutant allele

13 Reverse mutation-The production of a wild-type gene from a mutant gene

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15 Intragenic suppressor
Equivalent reversion UCC (Ser) forward UGC (Cys) reverse AGC (Ser) Wild type Mutant Wild type CGC (Arg, basic) forward CCC (Proline) reverse CAC (His, basic) Wild type Mutant Pseudo-wild type

16 Intragenic suppressor

17 Intergenic suppressor

18 Intergenic suppressor
Nonsense suppressor

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21 Chromosome transmission fidelity (Ctf) assay
non-essential Chromosome Fragment M SUP11 ade2-101 WT ade2-101 kar3D sic1D rad50D xrs2D CIN mutant ade1-101 ade2-101

22 Regulatory Coding

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26 A situation in which the third nucleotide
WOBBLE A situation in which the third nucleotide of an anticodon (at the 5’ end) can form two alignments. This third nucleotide can form hydrogen bonds not only with its normal complementary nucleotide in the third position but also with different nucleotide in the position.

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28 I stands for inosine, one of the rare bases found in tRNA, often in anticodon

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30 The genetic code is said to be degenerate because in many cases
MESSAGE The genetic code is said to be degenerate because in many cases more then one codon is assigned to a single amino acid, and, in addition, several codons can pair with more then on anticodon (wobble)


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