1. ANSWERS FOR QUIZ

2. Set-1. Answers to Bacterial gel
1.760
2. 245
3. 16 spots
4.142/760 x 100 i.e.18.68%
= 45.
6. 20 spots
7. 20/760 x 100 i.e.2.63%
= 35.
9.(i) Spot ID: 2082
pI : ~4.53
Mol. weight : 24.9 kDal
(ii) Spot ID: 1999
pI : ~4.90
Mol. weight : kDal

3. Supportive material

4. 1. Project Summary: Groups Control(1 image)
Project Name Bacterial sample 2D-Gels for Analysis.
Project Creation Date ………………
Author …………………
Description Bacterial sample Control vs Drug treated
Groups
Control(1 image)
780spots transferred from fused image
96 spots cancelled
Drug Treated(1 image)
780 spots transferred from fused image
Vector Matching :245

5. 3.Figure showing Molecular weight to pI
pI index 85
4.00
4.25
4.50
4.75
5.00
5.25
5.50
5.75
6.00
6.25
6.50
6.75
7.00 80 75 70 65 60 55 50 45 40 35 30 25 20 15 10 5
Molecular weight (kDal)
pI : ~4.53
Mol. weight : 24.9 kDal
pI : ~4.90
Mol. weight : kDal

6. 4.1.Differential expression : Up regulation
No. of folds
(and above)
No.of spots 2
142 4 71 6 36 8 26 10 16 12 15
Up regulation
x Folds or above
No. of spots showing up regulation

7. 4.2.Differential expression : Down regulation
No. of folds
(and above)
No.of spots 2
174 4 47 6 20 8 12 10 15 1
Down regulation
x Folds or above
No. of spots showing up regulation

8. Set -1. Answer of Serum sample
spots
2. 109
3. 16 spots
4. 69/349 x 100 i.e.19.77%
= 17
6. 28 spots
7. 48/349 x 100 i.e %
= 55
9.(i) Spot ID: 2082
pI : 5.28
Mol. weight : 44.8 kDal
(ii) Spot ID: 1999
pI : ~5.26
Mol. weight : kDal

9. Supportive material

10. Project Summary: Groups Control(1 image) Control - Healthy person
Project Name Serum sample 2D-Gels for Analysis.
Project Creation Date …………………………………
Author ……………….
Description Healthy vs diseased patient
Groups
Control(1 image)
Control - Healthy person
349spots transferred from fused image
97 spots cancelled
Diseased (1 image)
P.falciparum diseased serum sample
349 spots transferred from fused image
Vector Matching : 109

11. 3.Figure showing Molecular weight to pI
pI index 85
4.00
4.25
4.50
4.75
5.00
5.25
5.50
5.75
6.00
6.25
6.50
6.75
7.00 80 75 70 65 60 55 50 45 40 35 30 25 20 15 10 5
Molecular weight (kDal)
pI : 5.28
Mol. weight : kDal
pI : 5.26
Mol. weight : kDal

12. Differential expression : Up regulation
No. of folds
(and above)
No.of spots 2 69 3 47 4 39 5 33 8 21 10 16 15 20 25 50 75 1
Up regulation
x Folds or above
No. of spots showing up regulation

13. 4.2.Differential expression : Down regulation
No. of folds
(and above)
No. of spots 2 83 3 48 4 37 5 28 7 13 10 11 15 30
Down regulation
x Folds or above
No. of spots showing up regulation