Presentation is loading. Please wait.

Presentation is loading. Please wait.

Ultraviolet A Irradiation Induces NF-E2-Related Factor 2 Activation in Dermal Fibroblasts: Protective Role in UVA-Induced Apoptosis  Ayako Hirota, Yasuhiro.

Published byTeguh Hadiman Modified over 5 years ago

Similar presentations

Presentation on theme: "Ultraviolet A Irradiation Induces NF-E2-Related Factor 2 Activation in Dermal Fibroblasts: Protective Role in UVA-Induced Apoptosis  Ayako Hirota, Yasuhiro."— Presentation transcript:

1 Ultraviolet A Irradiation Induces NF-E2-Related Factor 2 Activation in Dermal Fibroblasts: Protective Role in UVA-Induced Apoptosis  Ayako Hirota, Yasuhiro Kawachi, Ken Itoh, Yasuhiro Nakamura, Xuezhu Xu, Tomohiro Banno, Takenori Takahashi, Masayuki Yamamoto, Fujio Otsuka  Journal of Investigative Dermatology  Volume 124, Issue 4, Pages (April 2005) DOI: /j X x Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

2 Figure 1 Ultraviolet A (UVA) increases both whole-cell and nuclear NF-E2-related factor 2 (Nrf2) accumulation in murine dermal fibroblasts. Fibroblasts were irradiated with UVA in phosphate-buffered saline and incubated for the indicated times. Nuclear extracts and whole-cell extracts were prepared as described in Materials and Methods. Equal amounts of protein were analyzed by SDS-PAGE and western blotting with anti-Nrf2 polyclonal antibody. (A) Nuclear extracts prepared 1, 2, and 4 h after UVA irradiation (0 or 10,000 mJ per cm2). (B) Nuclear extracts prepared at later time points (4, 6, and 8 h) after UVA irradiation at a dose of 0 or 10,000 mJ per cm2. (C) Nuclear extracts prepared after UVA irradiation at doses of 0, 5000, and 10,000 mJ per cm2. (D) Nuclear extracts and whole-cell extracts prepared 4 h after UVA irradiation (0 or 10,000 mJ per cm2). rNrf2, recombinant Nrf2 protein loaded as a molecular weight control. Antibody against lamin A/C was used as a loading control. Journal of Investigative Dermatology  , DOI: ( /j X x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

3 Figure 2 Ultraviolet B (UVB) decreases nuclear NF-E2-related factor 2 (Nrf2) accumulation in murine dermal fibroblasts. Fibroblasts were irradiated with UVB in phosphate-buffered saline and incubated for 4 h. Nuclear extracts were prepared as described in Materials and Methods. Equal amounts of protein were analyzed by SDS-PAGE and western blotting with anti-Nrf2 polyclonal antibody. (A) Nuclear extracts prepared 4 h after UVB irradiation at various doses (0, 15, 30, 60, 100, and 200 mJ per cm2). Antibody against lamin A/C was used as a loading control. (B) Nuclear extracts prepared 4 h after UVB irradiation at various doses (0, 2, 5, 10, and 15 mJ per cm2). (C) Nuclear extracts prepared 2, 4, and 6 h after UVB irradiation. Journal of Investigative Dermatology  , DOI: ( /j X x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

4 Figure 3 Nuclear accumulation of NF-E2-related Factor 2 (Nrf2) in response to ultraviolet A (UVA) irradiation. Cultured dermal fibroblasts were treated with or without UVA irradiation at a dose of 10,000 mJ per cm2. After 4 h of irradiation, cells were fixed and immunostained with anti-Nrf2 primary antibody followed by fluorescein isothiocyanate-labeled secondary antibody. (A) Non-irradiated fibroblasts; (B) UVA-irradiated fibroblasts. Journal of Investigative Dermatology  , DOI: ( /j X x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

5 Figure 4 Nuclear accumulation of NF-E2-related factor 2 (Nrf2) in the presence of hematoporphyrin in murine dermal fibroblasts. Fibroblasts were irradiated with ultraviolet A (UVA) (2000 mJ per cm2) in phosphate-buffered saline with hematoporphyrin and incubated in conventional medium for the indicated times. Nuclear extracts were prepared as described in Experimental Procedures. Equal amounts of protein were analyzed by SDS-PAGE and western blotting with anti-Nrf2 polyclonal antibody. Antibody against lamin A/C was used as a loading control. Journal of Investigative Dermatology  , DOI: ( /j X x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

6 Figure 5 Induction of γ-glutamylcysteine synthetase (γ-GCS) messenger RNA (mRNA) by ultraviolet A (UVA) irradiation. Total RNA were prepared from cultured dermal fibroblasts 5 h after exposure to UVA at a dose of 7000 mJ per cm2. Levels of mRNA for the typical Nrf2 target genes, i.e., γ-GCS heavy chain (GCSh; upper panel) and γ-GCS light chain (GCSl; middle panel), were determined by northern blotting analysis. The membranes were subsequently hybridized with a probe for the loading control (18S; lower panel). Journal of Investigative Dermatology  , DOI: ( /j X x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

7 Figure 6 Detection of ultraviolet A (UVA) radiation-induced cell death in NF-E2-related factor 2 (Nrf2)-deficient and wild-type murine dermal fibroblasts. Fibroblasts were irradiated with UVA at a dose of 15,000 mJ per cm2, incubated for 5 h in conventional medium, and labeled with annexin and propidium iodide (PI). The distributions of live and dead cells were determined by FACScalibur analysis of annexin and PI-labeled cells. Live cells were unlabeled with annexin and PI (R1), whereas annexin labeling (R2) represents the population undergoing apoptosis. Cells showing annexin and PI double labeling (R3) represent those that have already died by apoptosis. Ten thousand cells were analyzed in each sample. (A) Non-irradiated wild-type cells. (B) Non-irradiated nrf2-null mutant cells. (C) UVA-irradiated wild-type cells. (D) UVA-irradiated nrf2-null mutant cells. Journal of Investigative Dermatology  , DOI: ( /j X x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

8 Figure 7 Detection of ultraviolet A (UVA) radiation-induced cell death in Kelch-like-ECH-associated protein 1 (Keap1)-deficient and wild-type murine mouse dermal fibroblasts. Wild-type keap1- or keap1-deficient cells were treated and analyzed as described in Figure 6 except that the dose of UVA irradiation was 20,000 mJ per cm2. (A) Non-irradiated wild-type cells. (B) Non-irradiated keap1-null mutant cells. (C) UVA-irradiated wild-type cells. (D) UVA-irradiated keap1-null mutant cells. Journal of Investigative Dermatology  , DOI: ( /j X x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

Download ppt "Ultraviolet A Irradiation Induces NF-E2-Related Factor 2 Activation in Dermal Fibroblasts: Protective Role in UVA-Induced Apoptosis  Ayako Hirota, Yasuhiro."

Similar presentations

Glucocorticoids Augment the Chemically Induced Production and Gene Expression of Interleukin-1α through NF-κB and AP-1 Activation in Murine Epidermal.

Glucocorticoids Augment the Chemically Induced Production and Gene Expression of Interleukin-1α through NF-κB and AP-1 Activation in Murine Epidermal.
UVB Increases Urokinase-Type Plasminogen Activator Receptor (uPAR) Expression1  Christoph Marschall, Toshiko Nobutoh, Evelyn Braungart, Kathrin Douwes,

UVB Increases Urokinase-Type Plasminogen Activator Receptor (uPAR) Expression1  Christoph Marschall, Toshiko Nobutoh, Evelyn Braungart, Kathrin Douwes,
Importance of Tissue Transglutaminase in Repair of Extracellular Matrices and Cell Death of Dermal Fibroblasts After Exposure to a Solarium Ultraviolet.

Importance of Tissue Transglutaminase in Repair of Extracellular Matrices and Cell Death of Dermal Fibroblasts After Exposure to a Solarium Ultraviolet.
Keloid Fibroblasts Resist Ceramide-Induced Apoptosis by Overexpression of Insulin- Like Growth Factor I Receptor  Hiroshi Ishihara, Hiroshi Yoshimoto,

Keloid Fibroblasts Resist Ceramide-Induced Apoptosis by Overexpression of Insulin- Like Growth Factor I Receptor  Hiroshi Ishihara, Hiroshi Yoshimoto,
Proliferating Cultured Human Keratinocytes Are More Susceptible to Apoptosis Compared with Mouse Keratinocytes  Vijaya Chaturvedi, Patricia Bacon, Barbara.

Proliferating Cultured Human Keratinocytes Are More Susceptible to Apoptosis Compared with Mouse Keratinocytes  Vijaya Chaturvedi, Patricia Bacon, Barbara.
Effects of Betulinic Acid Alone and in Combination with Irradiation in Human Melanoma Cells  Edgar Selzer, Emilio Pimentel, Volker Wacheck, Werner Schlegel,

Effects of Betulinic Acid Alone and in Combination with Irradiation in Human Melanoma Cells  Edgar Selzer, Emilio Pimentel, Volker Wacheck, Werner Schlegel,
Ultraviolet Irradiation Induces Apoptosis in Human Immature, But Not in Skin Mast Cells  Sven Guhl, Silke Tapkenhinrichs, Alina Smorodchenko, Andreas Grützkau,

Ultraviolet Irradiation Induces Apoptosis in Human Immature, But Not in Skin Mast Cells  Sven Guhl, Silke Tapkenhinrichs, Alina Smorodchenko, Andreas Grützkau,
Expression of Type XVI Collagen in Human Skin Fibroblasts: Enhanced Expression in Fibrotic Skin Diseases  Atsushi Akagi, Shingo Tajima, Yutaka Nagai 

Expression of Type XVI Collagen in Human Skin Fibroblasts: Enhanced Expression in Fibrotic Skin Diseases  Atsushi Akagi, Shingo Tajima, Yutaka Nagai 
Phosphatidylinositol 3-Kinase/Akt-Dependent and -Independent Protection Against Apoptosis in Normal Human Melanocytes  Masahiro Oka, Akiko Kageyama, Mizuho.

Phosphatidylinositol 3-Kinase/Akt-Dependent and -Independent Protection Against Apoptosis in Normal Human Melanocytes  Masahiro Oka, Akiko Kageyama, Mizuho.
Expression of Indoleamine 2,3-Dioxygenase in Dermal Fibroblasts Functions as a Local Immunosuppressive Factor  Yunyuan Li, Edward E. Tredget, Ruhangiz.

Expression of Indoleamine 2,3-Dioxygenase in Dermal Fibroblasts Functions as a Local Immunosuppressive Factor  Yunyuan Li, Edward E. Tredget, Ruhangiz.
Role of Monocyte Chemoattractant Protein-1 and its Receptor,CCR-2, in the Pathogenesis of Bleomycin-Induced Scleroderma  Toshiyuki Yamamoto, Kiyoshi Nishioka 

Role of Monocyte Chemoattractant Protein-1 and its Receptor,CCR-2, in the Pathogenesis of Bleomycin-Induced Scleroderma  Toshiyuki Yamamoto, Kiyoshi Nishioka 
The DNA-Mismatch Repair Enzyme hMSH2 Modulates UV-B-Induced Cell Cycle Arrest and Apoptosis in Melanoma Cells  Markus Seifert, Stefan J. Scherer, Wilfried.

The DNA-Mismatch Repair Enzyme hMSH2 Modulates UV-B-Induced Cell Cycle Arrest and Apoptosis in Melanoma Cells  Markus Seifert, Stefan J. Scherer, Wilfried.
Regulation of UVB-Induced IL-8 and MCP-1 Production in Skin Keratinocytes by Increasing Vitamin C Uptake via the Redistribution of SVCT-1 from the Cytosol.

Regulation of UVB-Induced IL-8 and MCP-1 Production in Skin Keratinocytes by Increasing Vitamin C Uptake via the Redistribution of SVCT-1 from the Cytosol.
Jennifer L. Rizzo, Jessica Dunn, Adam Rees, Thomas M. Rünger 

Jennifer L. Rizzo, Jessica Dunn, Adam Rees, Thomas M. Rünger 
Keratinocyte-Releasable Stratifin Functions as a Potent Collagenase-Stimulating Factor in Fibroblasts  Aziz Ghahary, Feridoun Karimi-Busheri, Yvonne Marcoux,

Keratinocyte-Releasable Stratifin Functions as a Potent Collagenase-Stimulating Factor in Fibroblasts  Aziz Ghahary, Feridoun Karimi-Busheri, Yvonne Marcoux,
Non-Coherent Near Infrared Radiation Protects Normal Human Dermal Fibroblasts from Solar Ultraviolet Toxicity  Salatiel Menezes  Journal of Investigative.

Non-Coherent Near Infrared Radiation Protects Normal Human Dermal Fibroblasts from Solar Ultraviolet Toxicity  Salatiel Menezes  Journal of Investigative.
Decreased Growth Inhibitory Responses of Squamous Carcinoma Cells to Interferon-γ Involve Failure to Recruit cki Proteins into cdk2 Complexes  Beth L.

Decreased Growth Inhibitory Responses of Squamous Carcinoma Cells to Interferon-γ Involve Failure to Recruit cki Proteins into cdk2 Complexes  Beth L.
Regulation of IL-33 Expression by IFN-γ and Tumor Necrosis Factor-α in Normal Human Epidermal Keratinocytes  Jitlada Meephansan, Hidetoshi Tsuda, Mayumi.

Regulation of IL-33 Expression by IFN-γ and Tumor Necrosis Factor-α in Normal Human Epidermal Keratinocytes  Jitlada Meephansan, Hidetoshi Tsuda, Mayumi.
Ultraviolet Modulation of Human Macrophage Metalloelastase in Human Skin In Vivo  Jin Ho Chung, Jin Young Seo, Mi Kyoung Lee, Hee Chul Eun, Joo Heung Lee,

Ultraviolet Modulation of Human Macrophage Metalloelastase in Human Skin In Vivo  Jin Ho Chung, Jin Young Seo, Mi Kyoung Lee, Hee Chul Eun, Joo Heung Lee,
Green Tea Polyphenols Prevent Ultraviolet Light-Induced Oxidative Damage and Matrix Metalloproteinases Expression in Mouse Skin  Praveen K. Vayalil, Anshu.

Green Tea Polyphenols Prevent Ultraviolet Light-Induced Oxidative Damage and Matrix Metalloproteinases Expression in Mouse Skin  Praveen K. Vayalil, Anshu.

Similar presentations

Ads by Google