1. A Real-Time PCR Assay for the Simultaneous Detection of Functional N284I and L412F Polymorphisms in the Human Toll-Like Receptor 3 Gene 
Robert A. Brown, Raymund R. Razonable 
The Journal of Molecular Diagnostics 
Volume 12, Issue 4, Pages (July 2010)
DOI: /jmoldx
Copyright © 2010 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

2. Figure 1
Schematic representation of the LightCycler melting curve (on F2 channel) of a sample with a wild-type TLR3 851 AA gene that peaked at 62.47°C (solid dark line) and a homozygous gene variant 851TT that peaked at 56.86°C (dashed line). The flat line represents the control with no amplified TLR3 gene.
The Journal of Molecular Diagnostics  , DOI: ( /jmoldx )
Copyright © 2010 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

3. Figure 2
Representation of TLR3 gene sequencing for the A851T single nucleotide polymorphism. A: The wild-type gene containing AAC, which encodes for the amino acid aspargine. B: The A851T single nucleotide polymorphism with the codon ATC, which encodes for isoleucine.
The Journal of Molecular Diagnostics  , DOI: ( /jmoldx )
Copyright © 2010 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

4. Figure 3
Schematic representation of the LightCycler melting curve (F3 channel) of a wild-type TLR CC sample that peaked at 61.25°C (solid dark line), a homozygous variant 1234 TT that peaked at 57.98°C (dashed line), and a heterozygous sample containing one allele with 1234C and another allele with 1234T, thereby creating a melting curve with a double peak at both 57.98°C and 61.25°C (thin line). The flat line represents the control with no amplified TLR3.
The Journal of Molecular Diagnostics  , DOI: ( /jmoldx )
Copyright © 2010 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

5. Figure 4
Representation of TLR3 gene sequencing for the C1234T single nucleotide polymorphism. A: The wild-type gene containing the codon CTC, which encodes for the amino acid leucine. B: The heterozygous gene with one allele with the CTC codon and the second allele with the TTC codon, which encode for leucine and phenylalanine, respectively. C: The homozygous C1234T single nucleotide polymorphism, which contains the TTC codon encoding for phenylalanine.
The Journal of Molecular Diagnostics  , DOI: ( /jmoldx )
Copyright © 2010 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions