1. A B
100
PgHMGR1 98
EsHMGR 82
EuHMGR
SmHMGR
Plant 87
100
PgHMGR2 77
Motif 1
PqHMGR
100
Motif 2
CaHMGR
Motif 3
100
100
ScHMGR
AtHMGR1S
AtHMGR2
Fungi
PjHMGR
DmHMGR
Animal
100
HsHMGR
100
MmHMGR
0.1
Supplemental Figure S8. Structural features of PgHMGR1. A, A phylogenetic tree of HMGRs from various organisms including PgHMGR1 and PgHMGR2. Eleutherococcus senticosus (EsHMGR, AFM77981), Eucommia ulmoides (EuHMGR, AAV54051), Salvia miltiorrhiza (SmHMGR, ACD37361), Panax quinquefolius (PqHMGR, ACV65036), Camptotheca acuminata (CaHMGR, AAB69727), Solanum chacoense (ScHMGR, AEX26934), Arabidopsis thaliana (AtHMGR1S, At1g76490; AtHMGR2, At2g17370), fungi Pichia jadinii (O74164), and animals such as Homo sapiens (NP_000850), Drosophila melanogaster (P14773), and Mus musculus (NP_032281). The neighbor-joining method was used, and the branch lengths are proportional to the divergence, with the scale of 0.1 representing 10% changes. B, The conserved motifs among the members are highlighted in colored boxes with an arranged number, and the sequences of the motifs are depicted in Supplemental Figure S3.