1. Fig. 4. Features of PH in LLC1 lung tumor mice.
Features of PH in LLC1 lung tumor mice. (A) Schematic of the experimental design. C57BL/6 mice were intravenously injected with saline (WT) or LLC1 cells. After 10, 14, and 18 days from the initial injection, WT and LLC1 lung tumor–bearing mice underwent echocardiographic and physiological measurements. (B) Representative hematoxylin and eosin–stained sections of mouse lungs. Scale bar, 500 μm. (C) Echocardiographic measurements. (D) Physiological measurements of RVSP, SAP, PO2, and PVR of the mice. For WT, n = 9; for LLC1, n = 10. (E) Representative photomicrographs of vWF (brown)–stained and α-SMA (violet)–stained tissues, followed by (F) quantification of pulmonary vascular muscularization (given as a percentage for each range of vessel sizes). Scale bars, 20 μm. (G) Representative photomicrographs of elastica van Gieson–stained tissues, followed by (H) quantification of medial wall thickness of pulmonary vessels. Scale bars, 20 μm. (I) Representative photomicrographs of Sirius red staining (right). Scale bars, 20 μm. Quantitative image analysis of Sirius red staining (collagen deposition) in RV (left). For WT, n = 9; for LLC1, n = 10. *P < 0.05, ***P < compared with WT; §P < 0.05, §§P < 0.01, §§§P < compared with nontumor tissue of LLC1.
Soni Savai Pullamsetti et al., Sci Transl Med 2017;9:eaai9048
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