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Activation of T-Cells from Allergic Patients and Volunteers by p-Phenylenediamine and Bandrowski's Base  Eve Marie Coulter, Claire Jenkinson, Ying Wu,

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Presentation on theme: "Activation of T-Cells from Allergic Patients and Volunteers by p-Phenylenediamine and Bandrowski's Base  Eve Marie Coulter, Claire Jenkinson, Ying Wu,"— Presentation transcript:

1 Activation of T-Cells from Allergic Patients and Volunteers by p-Phenylenediamine and Bandrowski's Base  Eve Marie Coulter, Claire Jenkinson, Ying Wu, John Farrell, Brian Foster, Andrew Smith, Carolann McGuire, Camilla Pease, David Basketter, Clodagh King, Peter Simon Friedmann, Munir Pirmohamed, Brian Kevin Park, Dean J. Naisbitt  Journal of Investigative Dermatology  Volume 128, Issue 4, Pages (April 2008) DOI: /sj.jid Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

2 Figure 1 Scheme depicting proposed PPD oxidation.
Journal of Investigative Dermatology  , DOI: ( /sj.jid ) Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

3 Figure 2 Antigen-specific stimulation of lymphocytes from allergic patients, volunteers and cord bloods. Mean proliferative response of lymphocytes from (a) eight allergic patients and (b) 16 volunteers, stimulated with PPD and BB. (c) Maximum proliferation of PPD- and BB-stimulated lymphocytes isolated from patient and volunteer cohorts. (d) Comparison of BB-specific proliferation of peripheral blood lymphocytes from allergic patients (n=6) and volunteers (n=9) and cord blood lymphocytes (n=6). Coefficient of variation was consistently less than 20%. Journal of Investigative Dermatology  , DOI: ( /sj.jid ) Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

4 Figure 3 Antigen-specific stimulation of CD4+ T-cells from allergic patients and volunteers. Stimulation of CD4+ T cells from (a) allergic patients and (b) volunteers with PPD and/or BB. CD4+ and CD8+ T cells were depleted from peripheral blood before analysis of antigen stimulation using antibody-labeled magnetic beads. Data show one of three representative experiments using lymphocytes from different individuals. Coefficient of variation was consistently less than 20%. Journal of Investigative Dermatology  , DOI: ( /sj.jid ) Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

5 Figure 4 The relationship between cellular disposition of PPD and T-cell activation. (a) Radiometric HPLC traces showing the degradation of PPD culture, in the presence and absence of glutathione. (b) Comparison of the irreversible binding of PPD oxidation products to cellular (both patients and volunteers) and extracellular protein in the presence and absence of glutathione. (c) Rate of disappearance of PPD in the presence of patient and volunteer lymphocytes. (d) Proliferation of lymphocytes from four allergic patients pulsed with PPD in the presence and absence of glutathione for 24hours. Journal of Investigative Dermatology  , DOI: ( /sj.jid ) Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

6 Figure 5 Stimulation of T-cell clones from allergic patients and volunteers with PPD and/or BB. (a) Concentration-dependent proliferation of four representative PPD-specific (left-hand panel) and BB-specific (right-hand panel) T-cell clones. PPD-specific clones derive from four allergic patients, while BB-specific clones derive from two allergic patients and two volunteers. (b) PPD-specific T-cell clones from allergic patients do not cross-react with BB. Graph shows the maximum stimulation of PDD-specific T-cell clones with PPD and the absence of proliferation with BB (n=8; from four allergic patients). Coefficient of variation was consistently less than 20%. Journal of Investigative Dermatology  , DOI: ( /sj.jid ) Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

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