1. Volume 12, Issue 4, Pages 680-686 (October 2005)
Acute Lung Injury with Endotoxin or NO2 Does Not Enhance Development of Airway Epithelium from Bone Marrow 
Travis Beckett, Roberto Loi, Robert Prenovitz, Matthew Poynter, Kaarin K. Goncz, Benjamin T. Suratt, Daniel J. Weiss 
Molecular Therapy 
Volume 12, Issue 4, Pages (October 2005)
DOI: /j.ymthe
Copyright © 2005 The American Society of Gene Therapy Terms and Conditions

2. FIG. 1
Photo micrographs of hematoxylin and eosin-stained lung sections from (A) naïve (unirradiated) C57Bl/6 mouse lung 24 h after endotoxin administration, (B) naïve (unirradiated) C57Bl/6 mouse lung 48 h after NO2 administration, (C) female C57Bl/6 mouse 2 months after total body irradiation and transplantation of total marrow cells from male GFP transgenic mouse, (D) irradiated and transplanted mouse 1 month after NO2 administration (2 months after irradiation and transplantation), (E) irradiated and transplanted mouse 1 month after NO2 administration (2 months after irradiation and transplantation), and (F) naïve female mouse lung. Several features of acute lung injury are noted in A and B, including inflammatory cell infiltrates and alveolar wall thickening (arrows). Mouse lungs depicted in C–F display no obvious features of acute lung injury. Original magnification: 100×.
Molecular Therapy  , DOI: ( /j.ymthe )
Copyright © 2005 The American Society of Gene Therapy Terms and Conditions

3. FIG. 2
Photomicrographs of lung sections from female C57Bl/6 mice irradiated and transplanted with adult male donor cells and subsequently assessed either 2 (A, B) or 4 (C, D) months later. FISH for the Y chromosome is indicated in red and immunostaining for CD45 is indicated in blue. Green immunostaining indicates either Clara cell secretory protein (A, C) or pro-SPC (B, D). Nuclear staining in B is indicated in gray and by light blue arrows. Examples of specific donor-derived cells are indicated as follows: dark pink arrows, Y+CD45− cells in subairway interstitium (A, C); yellow arrows, Y+CD45+ donor-origin leukocytes; white arrows, Y+CD45− cells in the alveolar septa; orange arrow, Y+CD45−pro-SPC+ cell in the alveolar septa (B, D). Asterisks indicate region of magnified insets for each image. Samples are representative of sections from two mice per experimental condition. Original magnifications: A, D, 200×; B, C, 400×.
Molecular Therapy  , DOI: ( /j.ymthe )
Copyright © 2005 The American Society of Gene Therapy Terms and Conditions

4. FIG. 3
Photomicrographs of lung sections from irradiated chimeric female C57Bl/6 mice subsequently exposed to NO2 and assessed either 1 (A, B) or 3 (C, D) months later. FISH for the Y chromosome is indicated in red and immunostaining for CD45 is indicated in blue. Green immunostaining indicates cytokeratin (A), Clara cell secretory protein (C), or pro-SPC (B, D). Examples of specific donor-derived cells are indicated as follows: light pink arrows, Y+CK+ cells in airway and alveolar walls (A); yellow arrows, Y+CD45+ donor-origin leukocytes; dark pink arrows, Y+CD45− cells in the subairway interstitium (C); white arrow, Y+CD45− cell in the alveolar septa (B); orange arrow, Y+CD45−pro-SPC+ cell in the alveolar septa (B, D). Asterisks indicate region of magnified insets for each image. Samples are representative of sections from two mice per experimental condition. Original magnifications: A, B, C, 600×; D, 400×.
Molecular Therapy  , DOI: ( /j.ymthe )
Copyright © 2005 The American Society of Gene Therapy Terms and Conditions

5. FIG. 4
Photomicrographs of lung sections from irradiated chimeric female C57Bl/6 mice subsequently exposed to intranasal endotoxin (25 μg/mouse) and assessed either 1 (A, B) or 3 (C, D) months later. FISH for the Y chromosome is indicated in red and immunostaining for CD45 is indicated in blue. Green immunostaining indicates cytokeratin (A), pro-SPC (B, D), or CCSP (C). Examples of specific donor-derived cells are indicated as follows: light pink arrows, Y+CK+ cells in airway and alveolar walls (A); yellow arrows, Y+CD45+ donor-origin leukocytes; white arrows, Y+CD45− cell in the alveolar septa (B, D); orange arrows, Y+CD45−pro-SPC+ cell in the alveolar septa (B, D). Asterisks indicate region of magnified insets for each image. Samples are representative of sections from two mice per experimental condition. Original magnifications: A, 400×; B, D, 200×; C, 600×.
Molecular Therapy  , DOI: ( /j.ymthe )
Copyright © 2005 The American Society of Gene Therapy Terms and Conditions