1. Supplementation with estradiol-17β improves porcine oocyte maturation and subsequent embryo development 
Ji-Su Kim, Ph.D., Bong-Seok Song, Ph.D., Sang-Rae Lee, D.V.M., Ph.D., Seung-Bin Yoon, B.Sc., Jae-Won Huh, Ph.D., Sun-Uk Kim, Ph.D., Ekyun Kim, Ph.D., Sang-Hyun Kim, D.V.M., Ph.D., Young-Kug Choo, Ph.D., Deog-Bon Koo, Ph.D., Kyu-Tae Chang, Ph.D. 
Fertility and Sterility 
Volume 95, Issue 8, Pages (June 2011)
DOI: /j.fertnstert
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Effects of E2 on oocyte maturation and subsequent early embryogenesis in pigs. (A) Effects of E2 supplementation on meiotic progression of porcine oocytes at 22 and 44 hours of IVM (n = 3). (B, C) Expression level of p34cdc2 and phosphorylation of ERK1/2 in MII oocytes matured in the presence or absence of E2 (n = 3). (D, E) Effects of E2-assisted IVM on pronucleus formation and rates of fertilization and polyspermy after IVF (n = 3). (F, G) Effect of E2-assisted IVM on blastocyst development from IVF- or SCNT-derived porcine embryos (n = 4). (H, I) Effects of E2-assisted IVM on blastomere apoptosis (TUNEL) and the expression of apoptosis-associated genes in IVF- or SCNT-derived blastocysts (n ≥ 80). Data are shown as the mean ± SD. ∗Values differ significantly (P<.05).
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions