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Changes in gene expression patterns in the ureteric bud and metanephric mesenchyme in models of kidney development Robert O. Stuart, Kevin T. Bush, Sanjay K. Nigam Kidney International Volume 64, Issue 6, Pages (December 2003) DOI: /j x Copyright © 2003 International Society of Nephrology Terms and Conditions
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Figure 1 (A to E) Phase-contrast photomicrographs of isolated ureteric buds (UBs) cultured for 1 to 5days in BSN-CM supplemented with 10% fetal calf serum (FCS), 125ng/mL GDNF, and 250ng/mL fibroblast growth factor-1 (FGF-1). (F and G) Metanephric mesenchyme (MM) cocultured for up to 5days with a piece of embryonic spinal cord (SC) in media supplemented with 10% FCS. (G) Mesenchyme has been induced and the beginnings of differentiated tubules are evident. (H) Higher magnification of the area enclosed by box in (F). Comma-shaped body is highlighted by dashed line. Kidney International , DOI: ( /j x) Copyright © 2003 International Society of Nephrology Terms and Conditions
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Figure 2 Hierarchical clustering of differentially expressed genes from ureteric bud (UB) and metanephric mesenchyme (MM) together with genes from whole kidney development data. The genes were clustered in two dimensions according to their gene expression. Designations on the right side of clustergram refer to the tissue from which the genes were identified. Abbreviations are: K, kidney; e13, e15, e17, e19, day of embryonic gestation; nb, newborn; wk, 1week postpartum; sc, spinal cord; no, no spinal cord. Red represents up-regulated; blue represents down-regulated. Kidney International , DOI: ( /j x) Copyright © 2003 International Society of Nephrology Terms and Conditions
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Figure 3 Hierarchical clustering of differentially expressed genes from ureteric bud (UB) and metanephric mesenchyme (MM) from Figure 2. Whole kidney development data have been removed to allow for visualization of differences between MM and UB. Red represents up-regulated; blue represents down-regulated. Kidney International , DOI: ( /j x) Copyright © 2003 International Society of Nephrology Terms and Conditions
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