Presentation is loading. Please wait.

Presentation is loading. Please wait.

Pharmacologic relaxation of vein grafts is beneficial compared with pressure distention caused by upregulation of endothelial nitric oxide synthase and.

Published byBlanka Doležalová Modified over 5 years ago

Similar presentations

Presentation on theme: "Pharmacologic relaxation of vein grafts is beneficial compared with pressure distention caused by upregulation of endothelial nitric oxide synthase and."— Presentation transcript:

1 Pharmacologic relaxation of vein grafts is beneficial compared with pressure distention caused by upregulation of endothelial nitric oxide synthase and nitric oxide production  Ada W.Y. Chung, PhD, Pooja Rauniyar, BSc, Honglin Luo, MD, PhD, York N. Hsiang, MD, Cornelis van Breemen, DVM, PhD, Elena B. Okon, PhD  The Journal of Thoracic and Cardiovascular Surgery  Volume 132, Issue 4, Pages (October 2006) DOI: /j.jtcvs Copyright © 2006 The American Association for Thoracic Surgery Terms and Conditions

2 Figure 1 A, An illustration showing the beneficial effects of nitric oxide (NO). In the vasculature NO is generated from L-arginine by both constitutive and inducible NO synthase (endothelial NO synthase (eNOS) in endothelium and inducible NO synthase (iNOS) in smooth muscle cells (SMCs)). NO exerts its protective effects by inducing smooth muscle relaxation; inhibiting SMC proliferation, migration, and synthesis of extracellular matrix (ECM); inhibiting platelet and leukocyte activation and adhesion; and stimulating endothelial cells proliferation. B, We hypothesize that the replacement of pressure distention with pharmacologic vasodilatators in vein grafting preserves endothelium and NO formation in the vein graft. The augmented bioavailability of NO would be beneficial to suppress vascular remodeling and vein graft occlusion. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © 2006 The American Association for Thoracic Surgery Terms and Conditions

3 Figure 2 Vasomotor properties. A, Contraction of porcine undistended and distended veins in response to α-adrenergic stimulation (50 μmol/L phenylephrine [PE]) and acetylcholine (Ach)–induced relaxation (10 nmol/L to 3 μmol/L) and contraction induced by depolarization (80 mmol/L K+). B, Length-tension curves of an undistended (dotted line) and a distended (solid line) porcine vein. Results are representative of 6 similar experiments. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © 2006 The American Association for Thoracic Surgery Terms and Conditions

4 Figure 3 von Willebrand factor–stained cross-sections from undistended (control, A) and distended (B and C) veins indicating the presence of endothelial cells. Movat-stained sections showing the elastin fibril structure (black) in the undistended (D) and distended (E) veins. Scale bars = 0.05 mm. Representative images from 7 to 8 independent experiments are shown. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © 2006 The American Association for Thoracic Surgery Terms and Conditions

5 Figure 4 Regulation of gene transcription of endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS) in vein grafts prepared by pressure distention and pharmacologic vasodilatators. A, Representative polymerase chain reaction results showing the mRNA transcription of eNOS, iNOS, and β-actin (loading control). B, Bar chart is the densitometric measurement of iNOS transcription. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © 2006 The American Association for Thoracic Surgery Terms and Conditions

6 Figure 5 Regulation of nitric oxide synthase expression in vein grafts prepared by pressure distention and pharmacologic vasodilatators. Densitometric measurement of protein expressions of endothelial nitric oxide synthase (eNOS; A), inducible nitric oxide synthase (iNOS; B), and phospho-eNOS Ser1177 (C) is shown. Insets, representative immunoblots showing eNOS, iNOS phospho-eNOS Ser1177, and β-actin immunoreactivity. *P < .05. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © 2006 The American Association for Thoracic Surgery Terms and Conditions

7 Figure 6 Enzymatic activities of nitric oxide synthase (NOS) and concentrations of nitrate and nitrite determined by using a colorimetric assay. Bar charts are the averaged NOS activities (upper panel) and the sum of concentrations of nitrate and nitrite (lower panel). *P < .05. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © 2006 The American Association for Thoracic Surgery Terms and Conditions

Download ppt "Pharmacologic relaxation of vein grafts is beneficial compared with pressure distention caused by upregulation of endothelial nitric oxide synthase and."

Similar presentations

Urokinase treatment preserves endothelial and smooth muscle function in experimental acute arterial thrombosis  David Whitley, MD, Peter Gloviczki, MD,

Urokinase treatment preserves endothelial and smooth muscle function in experimental acute arterial thrombosis  David Whitley, MD, Peter Gloviczki, MD,
Effectiveness of combination of losartan potassium and doxycycline versus single-drug treatments in the secondary prevention of thoracic aortic aneurysm.

Effectiveness of combination of losartan potassium and doxycycline versus single-drug treatments in the secondary prevention of thoracic aortic aneurysm.
Vein wall re-endothelialization after deep vein thrombosis is improved with low- molecular-weight heparin  Daria K. Moaveni, MD, Erin M. Lynch, MS, Cathy.

Vein wall re-endothelialization after deep vein thrombosis is improved with low- molecular-weight heparin  Daria K. Moaveni, MD, Erin M. Lynch, MS, Cathy.
Dipeptidyl peptidase 4 inhibitor reduces intimal hyperplasia in rabbit autologous jugular vein graft under poor distal runoff  Akio Koyama, MD, Kimihiro.

Dipeptidyl peptidase 4 inhibitor reduces intimal hyperplasia in rabbit autologous jugular vein graft under poor distal runoff  Akio Koyama, MD, Kimihiro.
Wei Zhou, MD, Hong Chai, MD, PhD, Andy Courson, BS, Peter H

Wei Zhou, MD, Hong Chai, MD, PhD, Andy Courson, BS, Peter H
Early hypercholesterolemia contributes to vasomotor dysfunction and injury associated atherogenesis that can be inhibited by nitric oxide  Kathleen G.

Early hypercholesterolemia contributes to vasomotor dysfunction and injury associated atherogenesis that can be inhibited by nitric oxide  Kathleen G.
George D. Webb, PhD, Lay Har Lim, BSc, Vernon M. S

George D. Webb, PhD, Lay Har Lim, BSc, Vernon M. S
Improved vessel preservation after 4 days of cold storage: Experimental study in rat arteries  Birgit Zatschler, Peter Dieterich, PhD, Bianca Müller,

Improved vessel preservation after 4 days of cold storage: Experimental study in rat arteries  Birgit Zatschler, Peter Dieterich, PhD, Bianca Müller,
Long-acting oral phosphodiesterase inhibition preconditions against reperfusion injury in an experimental lung transplantation model  Eric S. Weiss, MD,

Long-acting oral phosphodiesterase inhibition preconditions against reperfusion injury in an experimental lung transplantation model  Eric S. Weiss, MD,
I know what the studies say, but what should I do for my patient?

I know what the studies say, but what should I do for my patient?
Endothelial nitric oxide synthase enhancer for protection of endothelial function from asymmetric dimethylarginine–induced injury in human internal thoracic.

Endothelial nitric oxide synthase enhancer for protection of endothelial function from asymmetric dimethylarginine–induced injury in human internal thoracic.
Interaction between neoplastic cells and cancer-associated fibroblasts through the CXCL12/CXCR4 axis: Role in non–small cell lung cancer tumor proliferation 

Interaction between neoplastic cells and cancer-associated fibroblasts through the CXCL12/CXCR4 axis: Role in non–small cell lung cancer tumor proliferation 
Reconstruction of pulmonary artery with porcine small intestinal submucosa in a lamb surgical model: Viability and growth potential  Lorenzo Boni, MD,

Reconstruction of pulmonary artery with porcine small intestinal submucosa in a lamb surgical model: Viability and growth potential  Lorenzo Boni, MD,
Pressure distention compared with pharmacologic relaxation in vein grafting upregulates matrix metalloproteinase-2 and -9  Ada W.Y. Chung, PhD, Pooja.

Pressure distention compared with pharmacologic relaxation in vein grafting upregulates matrix metalloproteinase-2 and -9  Ada W.Y. Chung, PhD, Pooja.
Daniel A. Langer, Vijay H. Shah  Journal of Hepatology 

Daniel A. Langer, Vijay H. Shah  Journal of Hepatology 
Circulating microparticles from patients with valvular heart disease and cardiac surgery inhibit endothelium-dependent vasodilation  Li Fu, MD, Xiao-Xia.

Circulating microparticles from patients with valvular heart disease and cardiac surgery inhibit endothelium-dependent vasodilation  Li Fu, MD, Xiao-Xia.
Dipeptidyl peptidase 4 inhibitor reduces intimal hyperplasia in rabbit autologous jugular vein graft under poor distal runoff  Akio Koyama, MD, Kimihiro.

Dipeptidyl peptidase 4 inhibitor reduces intimal hyperplasia in rabbit autologous jugular vein graft under poor distal runoff  Akio Koyama, MD, Kimihiro.
Postmortem and ex vivo carbon monoxide ventilation reduces injury in rat lungs transplanted from non–heart-beating donors  Boming Dong, MD, PhD, Paul.

Postmortem and ex vivo carbon monoxide ventilation reduces injury in rat lungs transplanted from non–heart-beating donors  Boming Dong, MD, PhD, Paul.
Techniques to harvest diseased human peripheral arteries and measure endothelial function in an ex vivo model  Ryan O. Lakin, MD, Weifei Zhu, PhD, Lindsay.

Techniques to harvest diseased human peripheral arteries and measure endothelial function in an ex vivo model  Ryan O. Lakin, MD, Weifei Zhu, PhD, Lindsay.
Adventitial delivery of platelet-derived endothelial cell growth factor gene prevented intimal hyperplasia of vein graft  Mitsuteru Handa, MD, Wei Li,

Adventitial delivery of platelet-derived endothelial cell growth factor gene prevented intimal hyperplasia of vein graft  Mitsuteru Handa, MD, Wei Li,

Similar presentations

Ads by Google