1. Title: Biology 4/10/07 Objectives: Class Topics
5/13/2019
Title: Biology 4/10/07
Objectives:
To review how proteins are formed and learn about the central dogma of biology.
Class Topics
Hand in W.S. How can a mutation in DNA affect an organism before the bell rings
Review Chapter 10 Quiz 1
Review Regulating Gene Production
Notes - Mutations
Editing RNA
Lab - Making Sense of Introns and Exons
Prepare for Chapter 10 Test
"I made it hard and difficult for kids because they don't get anything out of it if it's easy."
Bob Knight
Monday, May 13, :27 PM

2. Class Assignments Read 215-220 4/10/07
5/13/2019
Class Assignments
What
By When
Read /10/07
W.S. How can a mutation in DNA… 4/10/07
Making sense of introns and exons 4/10/07
Chapter 10 Test 4/12/07
Due this class period
Due next class period
Due in the future

3. 5/13/2019
Grade Sheet
2A – p. 157 (5 pts.)

4. From: http://www.accessexcellence.org/AB/GG/induction.html
5/13/2019
From:

5. From: http://www.accessexcellence.org/AB/GG/rna_synth.html
5/13/2019
From:

6. Mutations Gene alterations Involve the inclusion of a wrong amino acid
5/13/2019
Mutations
Gene alterations
Involve the inclusion of a wrong amino acid
Point mutation
1. Changing one nucleotide
Sickle cell anemia
Silent mutation (no change in amino acid)
2. Deletion
Loss of a nucleotide or segment of DNA
Frameshift mutation

7. From: http://waynesword.palomar.edu/transpos.htm
5/13/2019
Barbara McClintock
Insertion
A length of DNA is inserted into a gene
Transposon
“Jumping genes” – like cut and paste on computer
Genes that can move from one location on a chromosome to another
It can inactivate a gene or cause mutations
Transposons are in all organisms
Brings together different combinations of genes
Evolutionarily important

8. Intron and Exon Lab Working in pairs
5/13/2019
Intron and Exon Lab
Working in pairs
The older of the pair will do all evens and the younger will do all odds.
Hand in one sheet per group
Hand in the polypeptide phrase when complete and lab sheet
Return to your seat and prepare for exam next class period

9. Test Review RNA vs DNA Genetic code Types of RNA Transcription
5/13/2019
Test Review
RNA vs DNA
Genetic code
Universal
Codon
Types of RNA
mRNA
nRNA
tRNA
rRNA
Transcription
Translation
Gene Editing
Introns
Exons
Gene expression
lac operon
Mutation
Point
frameshift

10. Chapter 11 Genetic Engineering
5/13/2019
Chapter 11 Genetic Engineering
Genetic engineering
Manipulating genes for practical purposes
Recombinant DNA
DNA from two or more organisms
History
First used in 1973
Common today
Insulin, agriculture products?????

11. Steps in Genetic Engineering
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Steps in Genetic Engineering
1. Cutting DNA
Use restriction enzymes to cut gene and vector
Recognize specific sequences of DNA
Vector is something to move gene into cell
Usually virus, yeast, or plasmid (circular DNA in bacteria independent of main chromosome)
Leaves ‘sticky’ ends