1. A Protein Interface

2. Protein Folding
Protein
tRNA
Ribozome
mRNA

3. acetylcholine esterase
Binding Site
(Negative)
Acetylcholine
(positive)

4. Amino Acid Sequence of the enzyme: acetylcholine esterase
1 mfvnqrtrrp ymsvfvlvlg aavicpaygi idrlvvqtss gpirgrstmv qgrevhvfng
61 vpfakppvds lrfkkpvpae pwhgvldatr lppsciqery eyfpgfagee mwnpntnvse
121 dclylniwvp tktrlrhgrg lnfgsndyfq ddddfqrqhq skgglamlvw iygggfmsgt
181 stldiynaei laavgnviva smqyrvgafg flylapying yeedapgnmg mwdqalairw
241 lkenakafgg dpdlitlfge saggssvslh llspvtrgls krgilqsgtl napwshmtae
301 kalqiaegli ddcncnltml kespstvmqc mrnvdaktis vqqwnsysgi lgfpsaptid
361 gvfmtadpmt mlreanlegi dilvgsnrde gtyfllydfi dyfekdaats lprdkfleim
421 ntifnkasep ereaiifqyt gwesgndgyq nqhqvgravg dhfficptne falglterga
481 svhyyyfthr tstslwgewm gvlhgdevey ifgqpmnasl qyrqrerdls rrmvlsvsef
541 artgnpaleg ehwplytren piffifnaeg eddlrgekyg rgpmatscaf wndflprlra
601 wsvpskspcn lleqmsiasv sstmpivvmv vlvliplcaw wwaikknktp phpqviletr
661 afmh
GenBank Sequence

5. The Amino Acids
David L. Nelson and Michael M. Cox, Lehninger Principles of Biochemistry, 3rd edition, 2000, Worth Publishers, ISBN

6. Proteins Fold by Twisting Peptide Backbone

7. Protein Secondary Structure

8. Tertiary Structure (3-Dimensional Fold)
Motif
Simple combination of secondary structures
Appears in different proteins
Domain
More complex combination of secondary structures
Has a specific function

9. Predicting Folding Fold predictions are made by minimizing free energy
Hydrogen Bonds Decrease Free Energy
Some backbone angles are more stable than others