1. Volume 132, Issue 3, Pages 982-993 (March 2007)
Sex Steroid Regulation of Macrophage Migration Inhibitory Factor in Normal and Inflamed Colon in the Female Rat 
Eric Houdeau, Raphael Moriez, Mathilde Leveque, Christel Salvador–Cartier, Aurelie Waget, Lin Leng, Lionel Bueno, Richard Bucala, Jean Fioramonti 
Gastroenterology 
Volume 132, Issue 3, Pages (March 2007)
DOI: /j.gastro
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2. Figure 1
Tissue concentration of MIF (A), TNF-α (B), and IL-1β (C) in the normal colon throughout the estrous cycle. Each bar represents mean ± SEM of duplicate ELISA determinations performed in 6–8 animals per cycle stage. P < .05, ANOVA; aP < .05, Bonferonni’s post test; ns: not significant vs all stages.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2007 AGA Institute Terms and Conditions

3. Figure 2
Tissue concentration of MIF (A), TNF-α (B), and IL-1β (C) in the normal colon 7 days after ovariectomy. Each bar represents mean ± SEM of duplicate ELISA assays performed in 7 animals per group. ***P < .001 vs sham group; ns: not significant.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2007 AGA Institute Terms and Conditions

4. Figure 3
Effects of intracolonic administration of TNBS in either the follicular or the luteal phase of the estrous cycle on myeloperoxidase (MPO) activity in the colon. Values are mean ± SEM. ***P < .001 vs the corresponding saline control; aP < .01; ns: not significant between groups.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2007 AGA Institute Terms and Conditions

5. Figure 4
Immunohistochemical localization of MIF protein on transverse tissue sections in the noninflamed colon (A, C–F) and 24 hours after TNBS-induced colitis (G–H), as well as in the uterus (B) used as positive control for MIF immunoreactivity. (A) In cyclic rats without colitis, MIF positive labeling was mainly found in crypt epithelial cells (arrows), enteric ganglia (large arrowheads) and infiltrating immune cells in the lamina propria (small arrowheads). (B) Note that MIF positive staining on uterine tissue sections occurred in lumen epithelium (arrows) and endometrial glands (arrowhead), as established in other studies.36 (C–D) In the colon of EB-treated OVX rats, a general decrease of MIF staining intensities was found in colon epithelium (C) and enteric ganglia (arrowhead in D). (E–F) In P-treated OVX rats, MIF staining strongly increased at the base of crypts (arrows in E), surface epithelial cells, and enteric ganglia (arrowheads in F). (G–H) Twenty-four hours after TNBS, note the overall increase of MIF staining in the whole epithelial cells, not in enteric ganglia (arrowheads in H). lu: lumen; m: muscular wall; en: endometrium; ep: epithelium. All scale bars = 50 μm.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2007 AGA Institute Terms and Conditions

6. Figure 5
Influence of 17β-estradiol (EB) and progesterone (P) treatment on colonic (A) MIF, (B) TNF-α,(C) IL-1β concentrations, and (D) MPO activity in ovariectomized (OVX) rats with or without TNBS colitis. Values are means ± SEM of duplicate determinations performed in 6–9 animals per group. *P < .05, ** P < .01, *** P < .001; ns: not significant vs the corresponding control; aP < .05, bP < .01, and cP < .001 between groups.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2007 AGA Institute Terms and Conditions

7. Figure 6
Influence of 17β-estradiol (EB) and progesterone (P) treatment on (A) myeloperoxidase (MPO) activity and (B) MIF protein in the colon of ovariectomized (OVX) rats with or without DSS colitis. (A) Increased MPO activity at day 7 of DSS was significantly reduced by EB treatment, whereas P had no effect. (B) Western blot analysis of MIF protein in the colon (arrow), and quantitative representation of MIF protein content from 5 independent experiments. Values are mean ± SEM. **P < .01, ***P < .001; ns: not significant versus control; aP < .05 and bP < .001 between groups.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2007 AGA Institute Terms and Conditions

8. Figure 7
Effects of anti-MIF antibody on (A) MPO activity, (B) TNF-α concentration in the colon, and (C) plasma corticosterone in OVX rats with TNBS colitis under P impregnation. Values are mean ± SEM. *P < .05, **P < .01, ***P < .001; ns: not significant versus control; aP < .05, bP < .01, and cP < .001 between groups.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2007 AGA Institute Terms and Conditions