1. Only anti-CD133 antibodies recognizing the CD133/1 or the CD133/2 epitopes can identify human renal progenitors 
Maria L. Angelotti, Elena Lazzeri, Laura Lasagni, Paola Romagnani 
Kidney International 
Volume 78, Issue 6, Pages (September 2010)
DOI: /ki
Copyright © 2010 International Society of Nephrology Terms and Conditions

2. Figure 1
Only antibodies recognizing the CD133/1 or CD133/2 epitopes can identify human renal progenitors. (a, b) Double-label immunofluorescence performed with the anti-CD133 polyclonal antibody used by Ivanova et al. (pCD133, red) and the anti-CD133/2 monoclonal antibody (green) in embryonic human kidneys. The anti-CD133 antibody used by Ivanova et al. poorly stains primary vesicles, comma-shaped bodies and S-shaped bodies, while it extensively stains differentiated tubular cells as well as the differentiating ureteric bud (red). By contrast, the anti-CD133/2 antibody (green) selectively stains condensed mesenchyme-derived primordial structures, primary vesicles, comma-shaped bodies, S-shaped bodies and, in maturing glomeruli, the urinary pole of the Bowman's capsule (arrows). In (b), asterisk indicates a maturing glomerulus. Merged areas appear in yellow. Topro-3 counterstains nuclei (blue). Bar 100μm. (b′) Split image of the maturing glomerulus identified by the asterisk in (b). The arrow indicates the urinary pole of the Bowman's capsule. Bar 50μm (c) Double label immunofluorescence performed with the anti-CD133 polyclonal antibody used by Ivanova et al. (pCD133, red) and the anti-CD133/1 monoclonal antibody (green) in embryonic human kidneys. Only CD133/1 antibody selectively stains human progenitors of the Bowman's capsule (arrow). Merged areas appear in yellow. Topro-3 counterstains nuclei (blue). Bar 100μm.
Kidney International  , DOI: ( /ki )
Copyright © 2010 International Society of Nephrology Terms and Conditions