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Improving sample analysis and interpretation using QIAGEN’s latest Investigator® STR multiplex PCR assays with a novel quality sensor  M. Scherer, M.

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Presentation on theme: "Improving sample analysis and interpretation using QIAGEN’s latest Investigator® STR multiplex PCR assays with a novel quality sensor  M. Scherer, M."— Presentation transcript:

1 Improving sample analysis and interpretation using QIAGEN’s latest Investigator® STR multiplex PCR assays with a novel quality sensor  M. Scherer, M. König, M. Breitbach, S. Cornelius, M. Bussmann, A. Prochnow, R. Peist  Forensic Science International: Genetics Supplement Series  Volume 5, Pages e308-e309 (December 2015) DOI: /j.fsigss Copyright © 2015 Elsevier Ireland Ltd Terms and Conditions

2 Fig. 1 Quality sensor functionality data examples for Investigator 24plex QS purple channel are shown. Arrows indicate the presence or absence of QS fragments. (A) Amplification of 500pg control DNA (B) Amplification of 500pg control DNA 9948 in the presence of 1000μM hematin. (C) Amplification of 500pg control DNA 9948 in the absence of an essential assay component. (D) Amplification of 500pg DNA degraded to an average length of 150bp. Forensic Science International: Genetics Supplement Series 2015 5, e308-e309DOI: ( /j.fsigss ) Copyright © 2015 Elsevier Ireland Ltd Terms and Conditions

3 Fig. 2 Rework of an inhibited sample. Arrows indicate the presence or absence of QS fragments. (A) Amplification of 500pg control DNA 9948 in the presence of 1000μM hematin. (B) Amplification of 250pg control DNA 9948 in the presence of 500μM hematin. (C) Amplification of 1000pg control DNA 9948 in the presence of 2000μM hematin. Forensic Science International: Genetics Supplement Series 2015 5, e308-e309DOI: ( /j.fsigss ) Copyright © 2015 Elsevier Ireland Ltd Terms and Conditions


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